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Current Pharmaceutical Biotechnology


ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Research Article

Immunomodulatory Effects of Bifidobacterium longum W11 Produced Exopolysaccharide on Cytokine Production

Author(s): Rosanna Inturri*, Katia Mangano, Maria Santagati, Mariano Intrieri, Roberto Di Marco and Giovanna Blandino

Volume 18, Issue 11, 2017

Page: [883 - 889] Pages: 7

DOI: 10.2174/1389201019666171226151551

Price: $65


Background: Health benefits, including immune modulating capability, exerted by Bifidobacterium strains have been attributed to their exopolysaccharides (EPSs).

Objective: The effects of the purified EPS from B. longum W11 on cytokine production by peripheral blood mononuclear cells (PBMCs) alone or ConA-stimulated were investigated.

Method: The production of IFN-γ, IL-1β, IL-6 and IL-10 by PBMCs from healthy adult donors was analysed using purified EPS at two different concentrations (100 µg/mL and 200 µg/mL) and ConA, as an immunopotentiating marker. Moreover, the monosaccharide composition of the EPS from B. longum W11 was detected using HPLC analysis.

Results: The results demonstrated the ability of purified EPS to increase the production of the tested cytokines, except IL-10, in ConA-stimulated PBMCs. In not-stimulated-PBMCs, EPS increased the production of IL-6 (at 200 µg/mL) and IL-10 (at 100 µg/mL). The HPLC analysis showed the presence of main monomers, galactose and glucose (ratio 1:1 wt/wt), and small amount of rhamnose.

Conclusion: The results of this study demonstrate the ability of the EPS produced by B. longum W11 to interact in vitro with the human PBMCs, showing an immune-regulatory profile alone and an immune stimulatory profile in ConA-stimulated PBMCs. This suggests putative applications for the EPS from B. longum W11 in different pathological conditions.

Keywords: Bifidobacterium longum, probiotic, exopolysaccharide, chromatographic analysis, immunomodulatory effects, cytokine production, PBMC, HPLC.

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