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Current Protein & Peptide Science


ISSN (Print): 1389-2037
ISSN (Online): 1875-5550

Review Article

Viscosity Control of Protein Solution by Small Solutes: A Review

Author(s): Taehun Hong, Kazuki Iwashita and Kentaro Shiraki*

Volume 19, Issue 8, 2018

Page: [746 - 758] Pages: 13

DOI: 10.2174/1389203719666171213114919

open access plus


Viscosity of protein solution is one of the most troublesome issues for the high-concentration formulation of protein drugs. In this review, we summarize the practical methods that suppress the viscosity of protein solution using small molecular additives. The small amount of salts decreases the viscosity that results from electrostatic repulsion and attraction. The chaotrope suppresses the hydrophobic attraction and cluster formation, which can lower the solution viscosity. Arginine hydrochloride (ArgHCl) also suppresses the solution viscosity due to the hydrophobic and aromatic interactions between protein molecules. The small molecular additives are the simplest resolution of the high viscosity of protein solution as well as understanding of the primary cause in complex phenomena of protein interactions.

Keywords: Viscosity, arginine, antibody, immunoglobulin G, protein aggregation, serum albumin, molecular interaction.

Graphical Abstract

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