Monitoring Interactions Inside Cells by Advanced Spectroscopies: Overview of Copper Transporters and Cisplatin

Title:Monitoring Interactions Inside Cells by Advanced Spectroscopies: Overview of Copper Transporters and Cisplatin

Volume: 25 Issue: 4

Author(s): Alessia Lasorsa, Giovanni Natile*, Antonio Rosato, Francesco Tadini-Buoninsegni and Fabio Arnesano

Affiliation:

• Department of Chemistry, University of Bari “Aldo Moro”, Bari,Italy

Keywords: Platinum anticancer drugs, copper chaperones, copper ATPases, drug resistance, NMR spectroscopy, mass spectrometry.

Abstract: Background: Resistance, either at the onset of the treatment or developed after an initial positive response, is a major limitation of antitumor therapy. In the case of platinum- based drugs, copper transporters have been found to interfere with drug trafficking by facilitating the import or favoring the platinum export and inactivation.

Methods: The use of powerful spectroscopic, spectrometric and computational methods has allowed a deep structural insight into the mode of interaction of platinum drugs with the metal-binding domains of the transporter proteins.

Results: This review article focuses on the mode in which platinum drugs can compete with copper ion for binding to transport proteins and consequent structural and biological effects. Three types of transporters are discussed in detail: copper transporter 1 (Ctr1), the major responsible for Cu+ uptake; antioxidant-1 copper chaperone (Atox1), responsible for copper transfer within the cytoplasm; and copper ATPases (ATP7A/B), responsible for copper export into specific subcellular compartments and outside the cell.

Conclusion: The body of knowledge summarized in this review can help in shaping current chemotherapy to optimize the efficacy of platinum drugs (particularly in relation to resistance) and to mitigate adverse effects on copper metabolism.

Cite this article as:

Lasorsa Alessia, Natile Giovanni *, Rosato Antonio , Tadini-Buoninsegni Francesco and Arnesano Fabio , Monitoring Interactions Inside Cells by Advanced Spectroscopies: Overview of Copper Transporters and Cisplatin, Current Medicinal Chemistry 2018; 25 (4) . https://dx.doi.org/10.2174/0929867324666171110141311