The Regulation of Recombinant Protein S Secretion by Extracellular Factors

Title:The Regulation of Recombinant Protein S Secretion by Extracellular Factors

Volume: 12 Issue: 1

Author(s): Vijaya S. Pilli and Rinku Majumder*

Affiliation:

• LSU Health Science Center, Department of Biochemistry and Molecular Biology, New Orleans, LA-70112,United States

Keywords: Protein S, transcription, secretion, H2O2, CaCl2, glucose.

Abstract: Background: Protein S (PS) is a natural anticoagulant that has a vital function in regulating hemostasis and inflammation. Recently, the physiological and functional aspects of PS have gained considerable attention, whereas the biophysical properties of PS are far less well defined.

Objective: In this study, our goal was to improve the yield of recombinant PS with minimal contamination by endogenous, host cell PS.

Method: PS-stable cell lines were exposed to external factors and analyzed by qPCR and immunoblotting for endogenous and exogenous PS levels.

Results: Glucose treatment decreased the overall yield of endogenous and exogenous PS, hydrogen peroxide treatment increased the yield of both endogenous and exogenous PS, and calcium chloride treatment increased secretion of exogenous PS without affecting secretion of endogenous PS.

Conclusion: Supplementation of the expression medium with 5 mM calcium chloride improves the yield of recombinant PS.

Cite this article as:

Pilli S. Vijaya and Majumder Rinku *, The Regulation of Recombinant Protein S Secretion by Extracellular Factors, Current Chemical Biology 2018; 12 (1) . https://dx.doi.org/10.2174/2212796811666171109121623