Abstract
Fluorescence polarization technology has been used in basic research and commercial diagnostic assays for many decades, but has begun to be widely used in drug discovery only in the past six years. Originally, FP assays for drug discovery were developed for single-tube analytical instruments, but the technology was rapidly converted to high-throughput screening assays when commercial plate readers with equivalent sensitivity became available. This review will discuss fluorescence polarization assays in current use in drug discovery research as well as those in development that will likely be used in the near future. These assays include targets such as kinases, phosphatases, proteases, G-protein coupled receptors, and nuclear receptors.
Keywords: Fluorescence polarization, phosphatases, proteases, G-protein
Combinatorial Chemistry & High Throughput Screening
Title: Development and Application of Fluorescence Polarization Assays in Drug Discovery
Volume: 6 Issue: 3
Author(s): T. J. Burke, K. R. Loniello, J. A. Beebe and K. M. Ervin
Affiliation:
Keywords: Fluorescence polarization, phosphatases, proteases, G-protein
Abstract: Fluorescence polarization technology has been used in basic research and commercial diagnostic assays for many decades, but has begun to be widely used in drug discovery only in the past six years. Originally, FP assays for drug discovery were developed for single-tube analytical instruments, but the technology was rapidly converted to high-throughput screening assays when commercial plate readers with equivalent sensitivity became available. This review will discuss fluorescence polarization assays in current use in drug discovery research as well as those in development that will likely be used in the near future. These assays include targets such as kinases, phosphatases, proteases, G-protein coupled receptors, and nuclear receptors.
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Cite this article as:
Burke J. T., Loniello R. K., Beebe A. J. and Ervin M. K., Development and Application of Fluorescence Polarization Assays in Drug Discovery, Combinatorial Chemistry & High Throughput Screening 2003; 6 (3) . https://dx.doi.org/10.2174/138620703106298365
DOI https://dx.doi.org/10.2174/138620703106298365 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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