Glycan Phosphorylases in Multi-Enzyme Synthetic Processes

Title:Glycan Phosphorylases in Multi-Enzyme Synthetic Processes

Volume: 24 Issue: 8

Author(s): Giulia Pergolizzi, Sakonwan Kuhaudomlarp, Eeshan Kalita and Robert A. Field*

Affiliation:

• Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH,United Kingdom

Keywords: Phosphorylase, disaccharide, α-glucan, cellodextrin, high-value products, biofuel.

Abstract: Glycoside phosphorylases catalyse the reversible synthesis of glycosidic bonds by glycosylation with concomitant release of inorganic phosphate. The equilibrium position of such reactions can render them of limited synthetic utility, unless coupled with a secondary enzymatic step where the reaction lies heavily in favour of product. This article surveys recent works on the combined use of glycan phosphorylases with other enzymes to achieve synthetically useful processes.

Cite this article as:

Pergolizzi Giulia , Kuhaudomlarp Sakonwan , Kalita Eeshan and Field A. Robert *, Glycan Phosphorylases in Multi-Enzyme Synthetic Processes, Protein & Peptide Letters 2017; 24(8) . https://dx.doi.org/10.2174/0929866524666170811125109