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Current Drug Discovery Technologies


ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

Research Article

Flavonoid Enriched Fraction of Campylandra aurantiaca Attenuates Carbon Tetrachloride Induced Oxidative DNA Damage in Mouse Peritoneal Macrophages in Animal Model

Author(s): Mainak Chakraborty, Asis Bala* and Pallab Kanti Haldar

Volume 14, Issue 4, 2017

Page: [270 - 276] Pages: 7

DOI: 10.2174/1570163814666170504115137

Price: $65


Background: Recent studies have sought to draw attention of biological activity of Campylandra aurantiaca. The aim of the present study was to evaluate the effect of flavonoid enriched fraction of Campylandra aurantiaca (FEFCA) on in vitro and in vivo antioxidant and DNA protective effect in mouse peritoneal macrophages cells.

Methods: FEFCAwas characterized by HPLC analysis. The in vitro antioxidant activities of FEFCA was measured by different in vitro assays like 1, 1-diphenyl-2-picrylhydrazil radical (DPPH), superoxide anions, nitric oxide and hydroxyl radicals scavenging methods. Isolated mouse peritoneal macrophages were oxidized by carbon tetra chloride (CCl4) in animal model; subsequently the protective effect of FEFCA was determined in terms of estimation of antioxidant enzyme and the damage to DNA of the cells.

Results: FEFCA exhibited both in vitro antioxidant activities in a concentration dependent manner. FEFCA significantly (*p < 0.05) attenuated the oxidative DNA damage of mouse peritoneal macrophage cells induced by CCl4 in an in vivo assay.

Conclusion: Therefore FEFCA showed good free radical scavenging activity as well as reduced oxidative DNA damage in mouse peritoneal macrophages in animal model.

Keywords: Campylandra aurantiaca, flavonoid, oxidative stress, carbon tetra chloride, DNA protection, macrophages.

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