Fluorescence Assays for High-Throughput Screening of Protein Kinases

Title: Fluorescence Assays for High-Throughput Screening of Protein Kinases

Volume: 6 Issue: 4

Author(s): G. J.R. Zaman, A. Garritsen, Th. de Boer and C. A.A. van Boeckel

Affiliation:

Keywords: fluorescence assays, high-throughput screening, protein kinases, novel kinase inhibitors

Abstract: Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format. Here, we illustrate the application of fluorescence read-out technologies for kinase targets in light of our own experiences in assay development and high-throughput screening.

Cite this article as:

Zaman J.R. G., Garritsen A., Boer de Th. and van Boeckel A.A. C., Fluorescence Assays for High-Throughput Screening of Protein Kinases, Combinatorial Chemistry & High Throughput Screening 2003; 6 (4) . https://dx.doi.org/10.2174/138620703106298563