Phenotypic Screening of Small Molecule Libraries by High Throughput Cell Imaging

Title: Phenotypic Screening of Small Molecule Libraries by High Throughput Cell Imaging

Volume: 6 Issue: 4

Author(s): J. C. Yarrow, Y. Feng, Z. E. Perlman, T. Kirchhausen and T. J. Mitchison

Affiliation:

Keywords: high throughput cell imaging, high throughput fluorescence, chemical libraries, mitosis screen

Abstract: We have developed high throughput fluorescence cell imaging methods to screen chemical libraries for compounds with effects on diverse aspects of cell physiology. We describe screens for compounds that arrest cells in mitosis, that block cell migration, and that block the secretory pathway. Each of these screens yielded specific inhibitors for research use, and the mitosis screen identified Eg5 as a potential target protein for cancer chemotherapy. Cell imaging provides a large amount of information from primary screening data that can be used to distinguish compounds with different effects on cells, and together with automated analysis, to quantitate compound effects.

Cite this article as:

Yarrow C. J., Feng Y., Perlman E. Z., Kirchhausen T. and Mitchison J. T., Phenotypic Screening of Small Molecule Libraries by High Throughput Cell Imaging, Combinatorial Chemistry & High Throughput Screening 2003; 6 (4) . https://dx.doi.org/10.2174/138620703106298527