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Combinatorial Chemistry & High Throughput Screening


ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

Phenotypic Screening of Small Molecule Libraries by High Throughput Cell Imaging

Author(s): J. C. Yarrow, Y. Feng, Z. E. Perlman, T. Kirchhausen and T. J. Mitchison

Volume 6, Issue 4, 2003

Page: [279 - 286] Pages: 8

DOI: 10.2174/138620703106298527

Price: $65


We have developed high throughput fluorescence cell imaging methods to screen chemical libraries for compounds with effects on diverse aspects of cell physiology. We describe screens for compounds that arrest cells in mitosis, that block cell migration, and that block the secretory pathway. Each of these screens yielded specific inhibitors for research use, and the mitosis screen identified Eg5 as a potential target protein for cancer chemotherapy. Cell imaging provides a large amount of information from primary screening data that can be used to distinguish compounds with different effects on cells, and together with automated analysis, to quantitate compound effects.

Keywords: high throughput cell imaging, high throughput fluorescence, chemical libraries, mitosis screen

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