Nano pesticides are the best way to conventional pesticides. Nano pesticides can provide better penetration and accelerate resistance of microbes through the plant and control different diseases. Nano formulation of pesticides certainly protects the premature degradation in the environment and delivers high impact on targeted species. In this communication, we reported a novel nano-acephate for plant disease control and its subsequent characterization of encapsulated complex using polypropylene glycol as an encapsulated agent. Nano-acephate encapsulated particles were characterized by dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The bioactivity study was conducted against various fungi and bacteria. The performance of Nano particles was many fold times effective when compared to the original parental particles. The bio-assay of Nano acephate shows better results when compared to the normal commercial acephate. Less environmental contamination and safe handling are the advantages of nano-pesticides. This method is an eco-friendly formulation and it is useful to farmers.