A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection

Title:A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection

Volume: 17 Issue: 14

Author(s): Angel Montoya*, Carmen March, Yeison J. Montagut, Maria J. Moreno, Juan J. Manclus, Antonio Arnau, Yolanda Jimenez, Marisol Jaramillo, Paula A. Marin and Robinson A. Torres

Affiliation:

• Centro de Investigación e Innovación en Bioingeniería (Ci2B), Universitat Politècnica de València, Camino de Vera, s/n, 46022 Valencia,Spain

Keywords: Tuberculosis, 38 kDa Biomarker, Monoclonal Antibody, ELISA, High Fundamental Frequency (HFF), Piezoelectric Immunosensor.

Abstract: Background: Tuberculosis, one of the oldest diseases affecting human beings, is still considered as a world public health problem by the World Health Organization.

Method & Material: Therefore, there is a need for new and more powerful analytical methods for early illness diagnosis. With this idea in mind, the development of a High Fundamental Frequency (HFF) piezoelectric immunosensor for the sensitive detection of tuberculosis was undertaken. A 38 kDa protein secreted by Mycobacterium tuberculosis was first selected as the target biomarker. Then, specific monoclonal antibodies (MAbs) were obtained. Myc-31 MAb, which showed the highest affinity to the analyte, was employed to set up a reference enzyme-linked immunosorbent assay (ELISA) with a limit of detection of 14 ng mL-1 of 38 kDa antigen.

Results & Discussion: For the development of the HFF piezoelectric immunosensor, 100 MHz quartz crystals were used as transducer elements. The gold electrode surface was functionalized by covalent immobilization of the target biomarker through mixed self-assembled monolayers (mSAM) of carboxylic alkane thiols. A competitive immunoassay based on Myc-31 MAb was integrated with the transducer as sensing bio-recognition event. Reliable assay signals were obtained using low concentrations of antigen for functionalization and MAb for the competitive immunoassay. Under optimized conditions, the HFF immunosensor calibration curve for 38 kDa determination showed a limit of detection as low as 11 ng mL-1 of the biomarker. The high detectability attained by this immunosensor, in the picomolar range, makes it a promising tool for the easy, direct and sensitive detection of the tuberculosis biomarker in biological fluids such as sputum.

Cite this article as:

Montoya Angel*, March Carmen, Montagut J. Yeison, Moreno J. Maria, Manclus J. Juan, Arnau Antonio, Jimenez Yolanda, Jaramillo Marisol, Marin A. Paula and Torres A. Robinson, A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection, Current Topics in Medicinal Chemistry 2017; 17 (14) . https://dx.doi.org/10.2174/1568026617666161104105210

DOI https://dx.doi.org/10.2174/1568026617666161104105210	Print ISSN 1568-0266
Publisher Name Bentham Science Publisher	Online ISSN 1873-4294