摘要
背景:许多研究表明氧化应激是阿尔茨海默氏病(AD)的早期特征。然而, AD患者中的外周细胞的氧化应激的证据仍然是不确定的,原因可能有两个,样品类型的不同和研究中使用的氧化标记的异质性的差异。 本研究的目的是评估在阿尔茨海默氏病的血液为基础的氧化还原改变,以确定其特殊的疾病特点。 方法:为了达到目的,我们测定了AD, MCI 和对照组小鼠细胞外和细胞内的血室的超氧化物歧化酶,过氧化氢酶和谷胱甘肽过氧化物酶的活性。并氧化修饰导致的开放型p53蛋白量(unfolded p53)进行了测定。 结果:与对照组相比,AD, MCI小鼠中SOD降低、GPx活性和较高的开放型p53蛋白量增加。在外周血单个核细胞,与对照组相比,SOD活性在AD和MCI中下降,年轻男性p53蛋白量增加。 结论:总体而言,这些数据强调了为识别AD病理过程中特殊的以血液为基础的氧化还原改变,测定抗氧化酶活性以及特定的氧化终产物时,同时考虑细胞内外隔间的重要性。
关键词: 阿尔茨海默病,抗氧化酶,认知功能减退,轻度认知功能障碍,氧化还原变化,unfolded p53
Current Alzheimer Research
Title:Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients
Volume: 14 Issue: 1
Author(s): Noemí Arce-Varas, Giulia Abate, Chiara Prandelli, Carmen Martínez, Fernando Cuetos, Manuel Menéndez, Mariagrazia Marziano and David Cabrera-García, María Teresa Fernández-Sánchez, Antonello Novelli, Maurizio Memo, Daniela Uberti
Affiliation:
关键词: 阿尔茨海默病,抗氧化酶,认知功能减退,轻度认知功能障碍,氧化还原变化,unfolded p53
摘要: Background: Many studies suggest oxidative stress as an early feature of Alzheimer’s Disease (AD). However, evidence of established oxidative stress in AD peripheral cells is still inconclusive, possibly due to both, differences in the type of samples and the heterogeneity of oxidative markers used in different studies.
Objective: The aim of this study was to evaluate blood-based redox alterations in Alzheimer’s Disease in order to identify a peculiar disease profile. Method: To that purpose, we measured the activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase both in the extracellular and the intracellular blood compartments of AD, MCI and control subjects. The amount of an open isoform of p53 protein (unfolded p53), resulting from oxidative modifications was also determined. Results: Decreased SOD, increased GPx activity and higher p53 open isoform were found in both AD and MCI plasma compared to controls. In blood peripheral mononuclear cells, SOD activity was also decreased in both AD and MCI, and unfolded p53 increased exquisitely in younger AD males compared to controls. Conclusion: Overall, these data highlight the importance of considering both extracellular and intracellular compartments, in the determination of antioxidant enzyme activities as well as specific oxidation end-products, in order to identify peculiar blood-based redox alterations in AD pathology.Export Options
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Cite this article as:
Noemí Arce-Varas, Giulia Abate, Chiara Prandelli, Carmen Martínez, Fernando Cuetos, Manuel Menéndez, Mariagrazia Marziano and David Cabrera-García, María Teresa Fernández-Sánchez, Antonello Novelli, Maurizio Memo, Daniela Uberti , Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients, Current Alzheimer Research 2017; 14 (1) . https://dx.doi.org/10.2174/1567205013666161010125413
DOI https://dx.doi.org/10.2174/1567205013666161010125413 |
Print ISSN 1567-2050 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5828 |
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