Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients

Title:Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients

Volume: 14 Issue: 1

Author(s): Noemí Arce-Varas, Giulia Abate, Chiara Prandelli, Carmen Martínez, Fernando Cuetos, Manuel Menéndez, Mariagrazia Marziano, David Cabrera-García, María Teresa Fernández-Sánchez, Antonello Novelli, Maurizio Memo and Daniela Uberti

Affiliation:

Keywords: Alzheimer's disease, antioxidant enzymes, cognitive decline, mild cognitive impairment, redox alterations, unfolded p53.

Abstract: Background: Many studies suggest oxidative stress as an early feature of Alzheimer’s Disease (AD). However, evidence of established oxidative stress in AD peripheral cells is still inconclusive, possibly due to both, differences in the type of samples and the heterogeneity of oxidative markers used in different studies.

Objective: The aim of this study was to evaluate blood-based redox alterations in Alzheimer’s Disease in order to identify a peculiar disease profile.

Method: To that purpose, we measured the activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase both in the extracellular and the intracellular blood compartments of AD, MCI and control subjects. The amount of an open isoform of p53 protein (unfolded p53), resulting from oxidative modifications was also determined.

Results: Decreased SOD, increased GPx activity and higher p53 open isoform were found in both AD and MCI plasma compared to controls. In blood peripheral mononuclear cells, SOD activity was also decreased in both AD and MCI, and unfolded p53 increased exquisitely in younger AD males compared to controls.

Conclusion: Overall, these data highlight the importance of considering both extracellular and intracellular compartments, in the determination of antioxidant enzyme activities as well as specific oxidation end-products, in order to identify peculiar blood-based redox alterations in AD pathology.

Cite this article as:

Arce-Varas Noemí, Abate Giulia, Prandelli Chiara, Martínez Carmen, Cuetos Fernando, Menéndez Manuel, Marziano Mariagrazia, Cabrera-García David, Fernández-Sánchez Teresa María, Novelli Antonello, Memo Maurizio and Uberti Daniela, Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients, Current Alzheimer Research 2017; 14 (1) . https://dx.doi.org/10.2174/1567205013666161010125413

DOI https://dx.doi.org/10.2174/1567205013666161010125413	Print ISSN 1567-2050
Publisher Name Bentham Science Publisher	Online ISSN 1875-5828