Abstract
Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemists point of view.
Keywords: dna sequencing, single-molecule detection, dna polymerase, exonuclease, fluorescence
Current Pharmaceutical Biotechnology
Title: Optimal Enzymes for Single-Molecule Sequencing
Volume: 5 Issue: 1
Author(s): Susanne Brakmann
Affiliation:
Keywords: dna sequencing, single-molecule detection, dna polymerase, exonuclease, fluorescence
Abstract: Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemists point of view.
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Cite this article as:
Brakmann Susanne, Optimal Enzymes for Single-Molecule Sequencing, Current Pharmaceutical Biotechnology 2004; 5 (1) . https://dx.doi.org/10.2174/1389201043489611
DOI https://dx.doi.org/10.2174/1389201043489611 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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