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Current Pharmaceutical Analysis


ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Review Article

Methods to Evaluate the Activity of Nitric Oxide Synthase

Author(s): Marialuigia Fantacuzzi*, Alessandra Ammazzalorso, Barbara De Filippis, Letizia Giampietro, Cristina Maccallini and Rosa Amoroso

Volume 13, Issue 5, 2017

Page: [411 - 416] Pages: 6

DOI: 10.2174/1573412912666160916154405

Price: $65


Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented.

Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay.

Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of Lcitrulline as stoichiometric to nitric oxide.

Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.

Keywords: Nitric oxide synthase, nitric oxide, L-arginine, L-citrulline, griess assay, hemoglobin assay, citrulline assay, RPHPLC assay.

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