The Protective Effect of Jatrorrhizine Against Oxidative Stress in Primary Rat Cortical Neurons

Title:The Protective Effect of Jatrorrhizine Against Oxidative Stress in Primary Rat Cortical Neurons

Volume: 16 Issue: 5

Author(s): Tao Luo, Xiu-Yin Shen, Sheng Li, Ting Ouyang, Quan-An Mai*Hua-Qiao Wang*

Affiliation:

• Guangzhou Sport University, Guangzhou 510500,China

• Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, 510080,China

Keywords: Alzheimer's disease, apoptosis, Hydrogen peroxide, Jatrorrhizine, oxidative stress, neurons.

Abstract: Objectives: This study investigated the neuroprotective effects of Jatrorrhizine in rat cortical neurons.

Method: The effects of Jatrorrhizine on hydrogen peroxide (H₂O₂)-induced cell lesion, levels of lipid peroxidation and antioxidant enzyme activities were investigated in rat cortical neurons. Levels of mitochondrial membrane potential (MMP) and intracellular reactive oxygen species (ROS) were measured by fluorescent rhodamine staining and 2’,7’-dichlorfluorescein-diacetate staining, respectively. ATP content was measured by a high performance liquid chromatography. The protein levels for Bax, Bcl2 and cleaved caspase-3 were analyzed by western blot protein expression.

Results: There was a significant reduction in cell viability and activities of Superoxide dismutase and glutathione peroxidase for the cortical neurons after exposure to 50μM H₂O₂ for 12h. The hydrogen peroxide increased the production of malondialdehyde and ROS but decreased MMP and ATP in the neurons. However, pretreatment with different concentrations of Jatrorrhizine (5-20μM) inhibited H₂O₂-induced neurotoxicity markedly. Jatrorrhizine also attenuated the H₂O₂-induced Bcl-2/Bax ratio reduction and caspase-3 activation in these neurons.

Conclusions: Our findings suggest that Jatrorrhizine plays a critical neuroprotective role in H₂O₂ - induced apoptosis through its anti-oxidative actions. This may allow Jatrorrhizine to be a novel therapeutic with its high bioavailability to treat Alzheimer’s disease.

Cite this article as:

Luo Tao, Shen Xiu-Yin, Li Sheng, Ouyang Ting, Mai Quan-An *, Wang Hua-Qiao*, The Protective Effect of Jatrorrhizine Against Oxidative Stress in Primary Rat Cortical Neurons, CNS & Neurological Disorders - Drug Targets 2017; 16 (5) . https://dx.doi.org/10.2174/1871527315666160711101210

DOI https://dx.doi.org/10.2174/1871527315666160711101210	Print ISSN 1871-5273
Publisher Name Bentham Science Publisher	Online ISSN 1996-3181