Generic placeholder image

Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Review Article

Cell-Free Technologies for Proteomics and Protein Engineering

Author(s): Hiroyuki Ohashi and Etsuko Miyamoto-Sato

Volume 23, Issue 9, 2016

Page: [819 - 827] Pages: 9

DOI: 10.2174/0929866523666160613200304

open access plus


Cell-free translation systems facilitate rapid production of specific proteins and are particularly suited as high-throughput methods for whole-genome protein synthesis. Moreover, these systems do not rely on living cells, thereby allowing the synthesis of unstable or cytotoxic proteins in vitro. In this review, we describe the principles and potential applications of cell-free protein translation systems and the future prospects of proteomics approaches using next-generation sequencing and cell-free expression technologies.

Keywords: Cell-free, IVV (in vitro virus), mRNA display, PPI (protein-protein interaction), Proteomics, PURE system.

Graphical Abstract

© 2023 Bentham Science Publishers | Privacy Policy