Formulations were prepared containing Bergenia ligulata, Didymocarpus pedicellata, Dolichos biflorus, Boerhaavia diffusa, Crataeva nurvala and Ocimum sanctum in different ratios and coded as F1, F2 and F3. In vivo urolithiatic activities of the prepared formulations (i.e. F1:20, F1:40, F1:60, F2:20, F2:40, F2:60, F3:20, F3:40 and F3:60) were performed in rats using ethylene glycol induced urolithiasis model. It was observed that in comparison with the standard drug i.e. Cystone (30 mg/kg b wt.) the prepared formulation F3:60 (60 mg/kg b wt.) have shown significant antilithiatic activity.