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Current Stem Cell Research & Therapy


ISSN (Print): 1574-888X
ISSN (Online): 2212-3946

A Review of the Three-Dimensional Cell Culture Technique: Approaches, Advantages and Applications

Author(s): Weijie Zhang, Ai Zhuang, Ping Gu, Huifang Zhou and Xianqun Fan

Volume 11, Issue 4, 2016

Page: [370 - 380] Pages: 11

DOI: 10.2174/1574888X11666160217154007

Price: $65


Cell culture is a core and basic technique in biotechnology and is widely applied in biology, medicine, drug research and development. Traditional two-dimensional cell culture methods have undergone great developments. However, with in-depth basic research, higher requirements are needed to better mimic the in vivo environment to accurately observe cell behavior and explore its mechanisms. To comply with this situation, the three-dimensional cell culture technique emerged and has made profound advances in sustaining inherent cell properties. Here, we briefly review the development of this technique, including the main approaches to form three-dimensional microtissues, and its application and potential for future clinical therapies.

Keywords: Advantages, applications, scaffold, scaffold-free methods, three-dimensional cell culture.

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