摘要
尽管强大的新技术已经确定了许多新的人类和动物病毒,之前未知的病毒,如中东呼吸综合征冠状病毒不断涌现并威胁人类健康。同样的,现有的病毒如从埃博拉到基孔肯雅病毒,再度出现并传播到新的地理区域。由于其高致病性的研究,对这些病毒的研究往往是一项极具挑战的工作。慢病毒载体和假病毒是研究包膜病毒的良好工具,其促进了受体使用、病毒进入和血清学的研究进展。特别是,假病毒允许未知的或高致病性病毒的安全研究。他们还允许初始的感染特性如难培养病毒的细胞嗜性。本文将介绍了各种新兴病毒如基孔肯雅病毒、埃博拉病毒、SARS和MERS冠状病毒和尼帕病毒的假型系统,以及他们在不同的研究如药物筛选和抗体中和中的应用。我们同时也讨论了使用假病毒的限制和潜在的注意事项。
关键词: 假病毒,慢病毒载体,rhabdoviral载体,新型病毒,包膜病毒,血清学,药物筛选。
Current Gene Therapy
Title:Pseudotyping Viral Vectors With Emerging Virus Envelope Proteins
Volume: 16 Issue: 1
Author(s): Imke Steffen and Graham Simmons
Affiliation:
关键词: 假病毒,慢病毒载体,rhabdoviral载体,新型病毒,包膜病毒,血清学,药物筛选。
摘要: Previously unidentified viruses, such as Middle East respiratory syndrome coronavirus, continue to emerge and threaten populations, while powerful new techniques have identified many new human and animal viruses. Similarly, existing viruses, from Ebola virus to chikungunya virus, are reemerging and spreading to new geographical regions. These viruses often pose a challenge for researchers to study due to their highly pathogenic nature. Lentiviral and rhabdoviral pseudotypes are excellent tools for studying enveloped viruses and have contributed to many recent advances in areas such as receptor usage, viral entry and serology. In particular, pseudotypes allow the safe study of unknown or highly pathogenic viruses. They also allow the initial characterization of aspects of infection such as cellular tropism for difficult to culture viruses. In this review we will introduce various pseudotyping systems for emerging viruses, including chikungunya virus, Ebola virus, SARS and MERS coronaviruses and Nipah virus, as well as their use in diverse studies including drug screening and antibody neutralization. We will also discuss the limitations and potential caveats using pseudotypes.
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Cite this article as:
Imke Steffen and Graham Simmons , Pseudotyping Viral Vectors With Emerging Virus Envelope Proteins, Current Gene Therapy 2016; 16 (1) . https://dx.doi.org/10.2174/1566523216666160119093948
DOI https://dx.doi.org/10.2174/1566523216666160119093948 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
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