A Novel Quantitative Spectrophotometric Method for the Analysis of Vigabatrin in Pure Form and in Pharmaceutical Formulation

Title:A Novel Quantitative Spectrophotometric Method for the Analysis of Vigabatrin in Pure Form and in Pharmaceutical Formulation

Volume: 12 Issue: 4

Author(s): Majdi M. Bkhaitan and Agha Z. Mirza

Affiliation:

Keywords: Vigabatrin, ninhydrin, spectrophotomertic method, color complex, γ-aminobutyric acid, validation.

Abstract: Background: Vigabatrin is a variant of the inhibitory neurotransmitter γ-aminobutyric acid (GABA). It reduces seizure activities by inhibiting irreversibly GABA transaminase and increasing GABA concentrations.

Objective: This study presents simple, sensitive, extraction-free, and cost-efficient estimation of vigabatrin using a spectrophotometric method in pharmaceutical formulation.

Method: The method relies on the development of water-soluble, blue-violet colored complexes of vigabatrin and ninhydrin in a phosphate buffer of pH 7.4 with λ_max at 565 nm. Linearity, accuracy, precision, and specificity were performed as validation parameters for this method.

Results: Assessment of different analytical factors was carried out, and statistical analysis was performed to validate the results. The findings showed a linear progression in absorbance when the concentration of vigabatrin was increased. The correlation coefficient value (r²) of 0.9981 was observed. The operations followed the Beer’s law in the span of 40-200 µgmL^-1 with a molar absorptivity of 5.16x10³ Lmol^–1cm^–1. Recovery values in the marketed formulation of the assay method indicated no interference from the common additives and excipients. The limits of detection and quantification were found to be 6.0 and 40 µgmL^-1, respectively.

Conclusion: The presented method has demonstrated to be delicate, reliable, explicit, and intuitive that can prove beneficial for regular laboratory examination of vigabatrin in pharmaceutical dosage forms. '

Cite this article as:

Bkhaitan M. Majdi and Mirza Z. Agha, A Novel Quantitative Spectrophotometric Method for the Analysis of Vigabatrin in Pure Form and in Pharmaceutical Formulation, Current Pharmaceutical Analysis 2016; 12 (4) . https://dx.doi.org/10.2174/1573412912666151217182339