Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria

Title:Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria

Volume: 23 Issue: 1

Author(s): Malgorzata Slocińska, Grzegorz Rosinski and Wieslawa Jarmuszkiewicz

Affiliation:

Keywords: Insects, mitochondria, mitochondrial ATP-regulated channel (mKATP channel), superoxide production, uncoupling protein 4 (UCP4).

Abstract: It has been evidenced that mitochondrial uncoupling protein 4 (UCP4) and ATP-regulated potassium channel (mKATP channel) of insect Gromphadorhina coqereliana mitochondria decrease superoxide anion production. We elucidated whether the two energy-dissipating systems work together on a modulation of superoxide level in cockroach mitochondria. Our data show that the simultaneous activation of UCP4 by palmitic acid and mKATP channel by pinacidil revealed a cumulative effect on weakening mitochondrial superoxide formation. The inhibition of UCP4 by GTP (and/or ATP) and mKATP channel by ATP elevated superoxide production. These results suggest a functional cooperation of both energy-dissipating systems in protection against oxidative stress in insects.

Cite this article as:

Slocińska Malgorzata, Rosinski Grzegorz and Jarmuszkiewicz Wieslawa, Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria, Protein & Peptide Letters 2016; 23 (1) . https://dx.doi.org/10.2174/0929866523666151106121943