Optimization of the Microwave-Assisted Extraction of Azadirachta indica (Neem) Leaves Using NMR-based Metabolic Fingerprinting

Title:Optimization of the Microwave-Assisted Extraction of Azadirachta indica (Neem) Leaves Using NMR-based Metabolic Fingerprinting

Volume: 11 Issue: 3

Author(s): Simone Carradori, Luisa Mannina, Federica De Cosmi, Tamara Beccarini, Daniela Secci and Anatoly P. Sobolev

Affiliation:

Keywords: Azadirachta indica, leaves extract, microwave-assisted extraction, Neem, NMR.

Abstract: Neem (Azadirachta indica) leaves have been widely proposed as a traditional medicine and many bioactive compounds have been isolated and characterized. Taking into account the high variability of the constituents content, which is influenced by many factors such as the environment and the crop production technology, a proper and fast analytical method is important for monitoring the chemical composition of the product. Here, we report, for the first time, the NMR-based metabolic fingerprinting of Neem leaves extracts obtained using two different extraction techniques, namely microwave-assisted and conventional extractions. To optimize the recovery of a large range of metabolites, three extraction times (10, 30 and 60 minutes) and three solvents (methanol, chloroform and acetone) were used. The microwave-assisted extraction gave the highest recovery in the case of methanol and acetone, whereas conventional extraction was the best procedure for chloroform.

Cite this article as:

Carradori Simone, Mannina Luisa, De Cosmi Federica, Beccarini Tamara, Secci Daniela and P. Sobolev Anatoly, Optimization of the Microwave-Assisted Extraction of Azadirachta indica (Neem) Leaves Using NMR-based Metabolic Fingerprinting, Current Bioactive Compounds 2015; 11 (3) . https://dx.doi.org/10.2174/1573407211666151002001919