Abstract
Four stationary phases containing different groups such as: C18, C30, alkylamide, and cholesterolic, were presented for simultaneous HPLC analysis of structural isomers of tocopherol. Especially, the influence of stationary phase structure and properties on tuning of the highly selective HPLC separation of β- and γ- tocopherol pair demonstrating, respectively, para- and ortho- arrangement of methyl substituents on the 6- chromanol ring, has been elucidated. It was pointed out that selectivity of each stationary phase has been a result of modulation in the mass transfer and set of unspecific interactions in the tertiary system comprising analyte stationary phase mobile phase. Differences in observed retention and specific selectivity of tocopherols together with the stationary phase structure investigations indicated that a spatial organization changing of chemically bonded ligands as predominantly a solvation consequence. Additional molecular modeling studies preliminary explained some of these complicated supramolecular phenomena which caused that cholesterolic stationary phase offered beneficial performance in screening of tocopherols by HPLC and biomimetic studies of not completely recognized interactions of tocopherol isomers and biological membranes.
Keywords: stationary phases, liquid chromatography, tocopherols, selectivity tuning, hts
Combinatorial Chemistry & High Throughput Screening
Title: Effect of Stationary Phase Structure on Retention and Selectivity Tuning in the High-throughput Separation of Tocopherol Isomers by HPLC
Volume: 7 Issue: 4
Author(s): Boguslaw Buszewski, Katarzyna Krupczynska and Grzegorz Bazylak
Affiliation:
Keywords: stationary phases, liquid chromatography, tocopherols, selectivity tuning, hts
Abstract: Four stationary phases containing different groups such as: C18, C30, alkylamide, and cholesterolic, were presented for simultaneous HPLC analysis of structural isomers of tocopherol. Especially, the influence of stationary phase structure and properties on tuning of the highly selective HPLC separation of β- and γ- tocopherol pair demonstrating, respectively, para- and ortho- arrangement of methyl substituents on the 6- chromanol ring, has been elucidated. It was pointed out that selectivity of each stationary phase has been a result of modulation in the mass transfer and set of unspecific interactions in the tertiary system comprising analyte stationary phase mobile phase. Differences in observed retention and specific selectivity of tocopherols together with the stationary phase structure investigations indicated that a spatial organization changing of chemically bonded ligands as predominantly a solvation consequence. Additional molecular modeling studies preliminary explained some of these complicated supramolecular phenomena which caused that cholesterolic stationary phase offered beneficial performance in screening of tocopherols by HPLC and biomimetic studies of not completely recognized interactions of tocopherol isomers and biological membranes.
Export Options
About this article
Cite this article as:
Buszewski Boguslaw, Krupczynska Katarzyna and Bazylak Grzegorz, Effect of Stationary Phase Structure on Retention and Selectivity Tuning in the High-throughput Separation of Tocopherol Isomers by HPLC, Combinatorial Chemistry & High Throughput Screening 2004; 7 (4) . https://dx.doi.org/10.2174/1386207043328788
DOI https://dx.doi.org/10.2174/1386207043328788 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
Call for Papers in Thematic Issues
Artificial Intelligence Methods for Biomedical, Biochemical and Bioinformatics Problems
Recently, a large number of technologies based on artificial intelligence have been developed and applied to solve a diverse range of problems in the areas of biomedical, biochemical and bioinformatics problems. By utilizing powerful computing resources and massive amounts of data, methods based on artificial intelligence can significantly improve the ...read more
Eco-friendly Agents for Biological Control of Pathogenic Diseases
The discovery of an alternative biological approach to disease management includes work on medicinal products derived from natural sources as a starting point for the development of eco-friendly agents for these diseases and the injuries they cause, as well as reducing human contact with hazardous chemicals and their residues. We ...read more
Emerging trends in diseases mechanisms, noble drug targets and therapeutic strategies: focus on immunological and inflammatory disorders
Recently infectious and inflammatory diseases have been a key concern worldwide due to tremendous morbidity and mortality world Wide. Recent, nCOVID-9 pandemic is a good example for the emerging infectious disease outbreak. The world is facing many emerging and re-emerging diseases out breaks at present however, there is huge lack ...read more
Exploring Spectral Graph Theory in Combinatorial Chemistry
Scope of the Thematic Issue: Combinatorial chemistry involves the synthesis and analysis of a large number of diverse compounds simultaneously. Traditional methods rely on brute force experimentation, which can be time-consuming and resource-intensive. Spectral Graph Theory, a branch of mathematics dealing with the properties of graphs in relation to the ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Anti-Allergic Effects of Quercetin and Quercetin Liposomes in RBL-2H3
Cells
Endocrine, Metabolic & Immune Disorders - Drug Targets A Comprehensive Review on Fused Heterocyclic as DNA Intercalators: Promising Anticancer Agents
Current Pharmaceutical Design <sup>18</sup>F-Labeled Aryl-Tracers through Direct Introduction of [<sup>18</sup>F]fluoride into Electron-Rich Arenes
Current Organic Chemistry Application of 3-methylene- and 3-(monosubstituted)alkylideneoxindoles in the Synthesis of Heterocyclic Compounds
Current Organic Chemistry The Role of 3D Pharmacophore Mapping Based Virtual Screening for Identification of Novel Anticancer Agents: An Overview
Current Topics in Medicinal Chemistry Design, Synthesis, <i>In Silico</i> and <i>In Vitro</i> Evaluation of Novel Pyrimidine Derivatives as EGFR Inhibitors
Anti-Cancer Agents in Medicinal Chemistry Next Generation Biofilm Inhibitors for Pseudomonas aeruginosa: Synthesis and Rational Design Approaches
Current Topics in Medicinal Chemistry Reduction of Myocardial Ischemia-Reperfusion Injury with Pre- and Postconditioning: Molecular Mechanisms and Therapeutic Targets
Cardiovascular & Hematological Disorders-Drug Targets Mimics of Host Defense Proteins; Strategies for Translation to Therapeutic Applications
Current Topics in Medicinal Chemistry Pharmacogenetics of Irinotecan Disposition and Toxicity: A Review
Current Clinical Pharmacology A Risk Assessment Tool for the Virtual Screening of Metal Oxide Nanoparticles through Enalos InSilicoNano Platform
Current Topics in Medicinal Chemistry Receptor Guided 3D-QSAR Analysis of Thieno[2,3-b]Pyridine-5- Carbonitrile Inhibitors of Protein Kinase C Theta (PKC-θ )
Combinatorial Chemistry & High Throughput Screening Human- and Virus-Encoded microRNAs as Potential Targets of Antiviral Therapy
Mini-Reviews in Medicinal Chemistry COVID-19 and ARDS: Update on Preventive and Therapeutic Venues
Current Molecular Medicine Inhaled Corticosteroids and Pneumonia in Chronic Obstructive Pulmonary Disease
Current Respiratory Medicine Reviews Flavonoids: An Outstanding Structural Core for the Inhibition of Xanthine Oxidase Enzyme
Current Enzyme Inhibition ADAM Metalloproteinases as Potential Drug Targets
Current Medicinal Chemistry Chemokine Receptor CCR5 Antagonist Maraviroc: Medicinal Chemistry and Clinical Applications
Current Topics in Medicinal Chemistry Meet Our Editorial Board Member
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Perspective in Medicinal Chemistry: Structure-Based Drug Design
Current Topics in Medicinal Chemistry