Abstract
Mass spectrometry is a very versatile technique which has become both a high-throughput and a research tool in the biosciences. In this review, methods of functional protein analysis are discussed from a perspective in clinical research. The basics of proteomics is introduced as well as the determination of post-translational modifications such as phosphorylation. The investigation of protein interactions involves the measurement of non-covalent complexes and small molecule ligands as well as bioaffinity mass spectrometry. Comparative analysis of sample profiles on specific surfaces is performed increasingly to distinguish disease-related changes.
Keywords: matrix-assisted laser desorption (maldi), ion detector, magnetic sector, capillary electrophoresis, nanospray, phosphorylation analysis, ion microprobe, isotope-ratio mass spectrometry, proteomics, acetylation
Current Organic Chemistry
Title: Functional Protein Analysis Using Mass Spectrometry
Volume: 9 Issue: 9
Author(s): Simone Konig
Affiliation:
Keywords: matrix-assisted laser desorption (maldi), ion detector, magnetic sector, capillary electrophoresis, nanospray, phosphorylation analysis, ion microprobe, isotope-ratio mass spectrometry, proteomics, acetylation
Abstract: Mass spectrometry is a very versatile technique which has become both a high-throughput and a research tool in the biosciences. In this review, methods of functional protein analysis are discussed from a perspective in clinical research. The basics of proteomics is introduced as well as the determination of post-translational modifications such as phosphorylation. The investigation of protein interactions involves the measurement of non-covalent complexes and small molecule ligands as well as bioaffinity mass spectrometry. Comparative analysis of sample profiles on specific surfaces is performed increasingly to distinguish disease-related changes.
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Cite this article as:
Konig Simone, Functional Protein Analysis Using Mass Spectrometry, Current Organic Chemistry 2005; 9 (9) . https://dx.doi.org/10.2174/1385272054038291
| DOI https://dx.doi.org/10.2174/1385272054038291 |
Print ISSN 1385-2728 |
| Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
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