Abstract
Although the formation of the apolar core is crucial for protein folding, protein interior is often packed sub-optimally and incorporates water molecules that leave the bulk solvent. On average, one buried water molecules is observed every 20-90 amino acids, depending on the protein dimension and structural class: more buried water molecules are observed in large proteins and less in alpha proteins. From a structural perspective, it was shown that buried water molecules tend to be as rigid as buried protein atoms, incline to form hydrogen bonds with backbone residues of loops, and often are in contact with other buried water molecules. From a functional perspective, buried water molecules have a stabilizing effect by filling internal cavities and by interacting with polar atoms buried in the protein core and may also act as lubricants to favor loop dynamics. Their exchange kinetics with the bulk solvent is quite variable, ranging from few tens of nanoseconds to few tens of milliseconds.
Keywords: Crystallography, NMR spectroscopy, protein core, protein hydration, protein stability, protein structure, water.
Current Protein & Peptide Science
Title:Structure and Function of Water Molecules Buried in the Protein Core
Volume: 16 Issue: 3
Author(s): Oliviero Carugo
Affiliation:
Keywords: Crystallography, NMR spectroscopy, protein core, protein hydration, protein stability, protein structure, water.
Abstract: Although the formation of the apolar core is crucial for protein folding, protein interior is often packed sub-optimally and incorporates water molecules that leave the bulk solvent. On average, one buried water molecules is observed every 20-90 amino acids, depending on the protein dimension and structural class: more buried water molecules are observed in large proteins and less in alpha proteins. From a structural perspective, it was shown that buried water molecules tend to be as rigid as buried protein atoms, incline to form hydrogen bonds with backbone residues of loops, and often are in contact with other buried water molecules. From a functional perspective, buried water molecules have a stabilizing effect by filling internal cavities and by interacting with polar atoms buried in the protein core and may also act as lubricants to favor loop dynamics. Their exchange kinetics with the bulk solvent is quite variable, ranging from few tens of nanoseconds to few tens of milliseconds.
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Cite this article as:
Carugo Oliviero, Structure and Function of Water Molecules Buried in the Protein Core, Current Protein & Peptide Science 2015; 16 (3) . https://dx.doi.org/10.2174/1389203716666150227162803
DOI https://dx.doi.org/10.2174/1389203716666150227162803 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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