Abstract
In this research, greener chromatography employing a more eco-friendly microemulsion mobile phase was utilized for the analysis of rosiglitazone (ROS) and glimepiride (GLM) in rat sera. ROS, GLM, and darfenacin (an internal standard) were detected using UV wavelength of 237 nm. HPLC analysis by using a monolithic column was performed by directly injecting the sample after appropriate dilution with the mobile phase. The chromatographic behavior of these compounds was studied to demonstrate their chromatographic efficiency, retention, and peak symmetry. Regression analyses showed excellent linearity (r = 0.999) over concentrations ranging from 100 to 5000 ng mL-1for both ROS and GLM. The developed method by using rat sera had limits of detection of 40.0 ng mL-1for both drugs. The assay was statistically validated for linearity, accuracy, precision and specificity according to ICH guidelines. The intra- and inter-assay precisions were satisfactory and the accuracy of the method was proved. This assay was successfully applied in a pharmacokinetic study.
Keywords: Green chromatography, monolithic column, rosiglitazone and glimepiride, rat sera, pharmacokinetics.
Current Analytical Chemistry
Title:Greener Liquid Chromatography for Simultaneous Determination of Rosiglitazone and Glimepiride in Rat Sera and its Application to a Pharmacokinetic Study
Volume: 11 Issue: 3
Author(s): Mohamed Hefnawy, Nasser Algrain, Mohammed Abounassif, Sabry Attia and Yasser El-Shabrawy
Affiliation:
Keywords: Green chromatography, monolithic column, rosiglitazone and glimepiride, rat sera, pharmacokinetics.
Abstract: In this research, greener chromatography employing a more eco-friendly microemulsion mobile phase was utilized for the analysis of rosiglitazone (ROS) and glimepiride (GLM) in rat sera. ROS, GLM, and darfenacin (an internal standard) were detected using UV wavelength of 237 nm. HPLC analysis by using a monolithic column was performed by directly injecting the sample after appropriate dilution with the mobile phase. The chromatographic behavior of these compounds was studied to demonstrate their chromatographic efficiency, retention, and peak symmetry. Regression analyses showed excellent linearity (r = 0.999) over concentrations ranging from 100 to 5000 ng mL-1for both ROS and GLM. The developed method by using rat sera had limits of detection of 40.0 ng mL-1for both drugs. The assay was statistically validated for linearity, accuracy, precision and specificity according to ICH guidelines. The intra- and inter-assay precisions were satisfactory and the accuracy of the method was proved. This assay was successfully applied in a pharmacokinetic study.
Export Options
About this article
Cite this article as:
Hefnawy Mohamed, Algrain Nasser, Abounassif Mohammed, Attia Sabry and El-Shabrawy Yasser, Greener Liquid Chromatography for Simultaneous Determination of Rosiglitazone and Glimepiride in Rat Sera and its Application to a Pharmacokinetic Study, Current Analytical Chemistry 2015; 11 (3) . https://dx.doi.org/10.2174/1573411011666150219203414
DOI https://dx.doi.org/10.2174/1573411011666150219203414 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Prenatal Exposure of a Novel Antipsychotic Aripiprazole: Impact on Maternal, Fetal and Postnatal Body Weight Modulation in Rats
Current Drug Safety SGLT2 Inhibitors in Diabetes Mellitus Treatment
Reviews on Recent Clinical Trials Chromogranin-A: A Multifaceted Cardiovascular Role in Health and Disease
Current Medicinal Chemistry Sympathetic Overactivity in Patients with Chronic Renal Failure - The Culprit of Increased Cardiovascular Mortality?
Current Hypertension Reviews The Genetic Basis of Glucose Homeostasis
Current Diabetes Reviews Obesity Related Kidney Disease
Current Diabetes Reviews Effect of Metformin Therapy on Serum Fetuin Levels in Insulin Resistant Type 1 Diabetics
Current Diabetes Reviews Basic and Clinical Aspects of Gene Therapy for Retinopathy Induced by Diabetes
Current Gene Therapy Structural Basis for the Design of PPAR-γ Ligands: A Survey on Quantitative Structure- Activity Relationships
Mini-Reviews in Medicinal Chemistry Diabetes Mellitus and Osteoporosis Correlation: Challenges and Hopes
Current Diabetes Reviews The Heme Oxygenase/Biliverdin Reductase Pathway in Drug Research and Development
Current Drug Metabolism Amyloid and Tau Positron Emission Tomography in Suggested Diabetesrelated Dementia
Current Alzheimer Research Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 (LOX-1), a Relevant Target for Diabetic Vasculopathy?
Cardiovascular & Hematological Disorders-Drug Targets Patent Selections
Recent Patents on Inflammation & Allergy Drug Discovery Dietary Toxins, Endoplasmic Reticulum (ER) Stress and Diabetes
Current Diabetes Reviews Synchronized Fast SPE and UFLC Methods for the Analyses of Eight Antidiabetic Drugs in Human Plasma
Combinatorial Chemistry & High Throughput Screening Sympathetic Overactivity in Hypertension and Cardiovascular Disease
Current Vascular Pharmacology Pathophysiology of Atherosclerosis: The Role of Inflammation
Current Pharmaceutical Design Efficacy and Safety Outcomes of Short Duration Antiplatelet Therapy with Early Cessation of Aspirin Post Percutaneous Coronary Intervention: A Systematic Review and Meta-analysis
Current Cardiology Reviews Cholinesterase Inhibitors Modulate Autonomic Function in Patients with Alzheimer´s Disease and Mixed Dementia
Current Alzheimer Research