Abstract
A peculiar population of glial cells, Olfactory Ensheathing Cells (OECs), are able to support the continuous neuronal turn-over and sheathe olfactory axons. In vitro, they stimulate axonal growth, as produce several neurotrophic factors (GFs); in vivo they promote remyelination of damaged axons. In this in vitro study, OEC effects on survival of cortical neurons exposed to hypoxia were examined. Rat co-cultures of OECs and cortical neurons were placed both in normal and hypoxic conditions; subsequently cells were analyzed by immunocytochemistry. Furthermore, some neuronal cultures were grown with Glial cell Derived Neurotrophic Factor (GDNF) or basic Fibroblast Growth Factor (bFGF) to tentatively rescue cells from oxygen deprivation. Some cortical neurons grown in both conditions were considered as control cells. Some neuronal cultures were feed with conditioned medium from OECs. We show that both in co-cultures and with GFs-treatment there was an increase of the number of neurons in comparison with control cultures. Moreover, these neurons formed a rich axonal outgrowth. OEC-conditioned media did not affect the cell survival. In hypoxic cultures the neuron number was very low both in controls and in GFs-treated neurons, while in co-cultures and in OEC-conditioned media cultures an increased neuronal survival was observed. These data suggest that OECs promote the survival of neurons in vitro exposed to hypoxia exerting a protective influence. Since some experiments in vivo have shown that injury is often characterized by secondary insults, ischemia or hypoxia, our results suggest that OECs might be considered a possible approach for restoration in injuries.
Keywords: Cell viability, cortical neurons, growth factors, hypoxia, immunocytochemistry, neuroprotective effect, olfactory ensheathing cells.
CNS & Neurological Disorders - Drug Targets
Title:Olfactory Ensheathing Cells Protect Cortical Neuron Cultures Exposed to Hypoxia
Volume: 14 Issue: 1
Author(s): Rosalia Pellitteri, Antonella Russo, Stefania Stanzani and Damiano Zaccheo
Affiliation:
Keywords: Cell viability, cortical neurons, growth factors, hypoxia, immunocytochemistry, neuroprotective effect, olfactory ensheathing cells.
Abstract: A peculiar population of glial cells, Olfactory Ensheathing Cells (OECs), are able to support the continuous neuronal turn-over and sheathe olfactory axons. In vitro, they stimulate axonal growth, as produce several neurotrophic factors (GFs); in vivo they promote remyelination of damaged axons. In this in vitro study, OEC effects on survival of cortical neurons exposed to hypoxia were examined. Rat co-cultures of OECs and cortical neurons were placed both in normal and hypoxic conditions; subsequently cells were analyzed by immunocytochemistry. Furthermore, some neuronal cultures were grown with Glial cell Derived Neurotrophic Factor (GDNF) or basic Fibroblast Growth Factor (bFGF) to tentatively rescue cells from oxygen deprivation. Some cortical neurons grown in both conditions were considered as control cells. Some neuronal cultures were feed with conditioned medium from OECs. We show that both in co-cultures and with GFs-treatment there was an increase of the number of neurons in comparison with control cultures. Moreover, these neurons formed a rich axonal outgrowth. OEC-conditioned media did not affect the cell survival. In hypoxic cultures the neuron number was very low both in controls and in GFs-treated neurons, while in co-cultures and in OEC-conditioned media cultures an increased neuronal survival was observed. These data suggest that OECs promote the survival of neurons in vitro exposed to hypoxia exerting a protective influence. Since some experiments in vivo have shown that injury is often characterized by secondary insults, ischemia or hypoxia, our results suggest that OECs might be considered a possible approach for restoration in injuries.
Export Options
About this article
Cite this article as:
Pellitteri Rosalia, Russo Antonella, Stanzani Stefania and Zaccheo Damiano, Olfactory Ensheathing Cells Protect Cortical Neuron Cultures Exposed to Hypoxia, CNS & Neurological Disorders - Drug Targets 2015; 14 (1) . https://dx.doi.org/10.2174/1871527314666150116121105
DOI https://dx.doi.org/10.2174/1871527314666150116121105 |
Print ISSN 1871-5273 |
Publisher Name Bentham Science Publisher |
Online ISSN 1996-3181 |
Call for Papers in Thematic Issues
Diagnosis and treatment of central nervous system infectious diseases
Infectious diseases of the central nervous system (CNS) can be divided into bacterial, tuberculous, viral, fungal, parasitic infections, etc. Early etiological treatment is often the most crucial means to reduce the mortality rate of patients with central nervous system infections, reduce complications and sequelae, and improve prognosis. The initial clinical ...read more
Techniques of Drug Repurposing: Delivering a new life to Herbs & Drugs
Of late, with the adaptation of innovative approaches and integration of advancements made towards medical sciences as well as the availability of a wide range of tools; several therapeutic challenges are being translated into viable clinical solutions, with a high degree of efficacy, safety, and selectivity. With a better understanding ...read more
Trends and perspectives in the rational management of CNS disorders
Central nervous system (CNS) diseases enforce a significant global health burden, driving ongoing efforts to improve our understanding and effectiveness of therapy. This issue investigates current advances in the discipline, focusing on the understanding as well as therapeutic handling of various CNS diseases. The issue covers a variety of diseases, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Antifungal Azole Derivatives and their Pharmacological Potential: Prospects & Retrospects
The Natural Products Journal SV40 and HIV Sequences in the Cerebrospinal Fluid of a Patient with AIDS Dementia Complex
Current HIV Research Role of Defensins and Cathelicidin LL37 in Auto-Immune and Auto- Inflammatory Diseases
Current Pharmaceutical Biotechnology Ceftriaxone-Vancomycin Drug Toxicity Reduction by VRP 1020 in Mus musculus Mice
Current Clinical Pharmacology Ghetto Poverty and Pollution in Egypt: A Deadly Threat for Western Countries Caused by New and Infectious Mutants. A Cultural, Social and Microbiological Synopsis
Inflammation & Allergy - Drug Targets (Discontinued) Sulfonamide Agents for Treatment of Staphylococcus MRSA and MSSA Infections of the Central Nervous System
Central Nervous System Agents in Medicinal Chemistry Angiopoietin-1 and C16 Peptide Attenuate Vascular and Inflammatory Responses in Experimental Allergic Encephalomyelitis
CNS & Neurological Disorders - Drug Targets TLR2 and TLR4 Expression During Bacterial Infections
Current Pharmaceutical Design The Multimodal Treatment of Acromegaly: Current Status and Future Perspectives
Endocrine, Metabolic & Immune Disorders - Drug Targets Seroprevalence of Hepatitis E Virus Infection Among Pregnant Women in Ilam, West of Iran
Infectious Disorders - Drug Targets The Molecular Basis of Susceptibility to Infection in Liver Cirrhosis
Current Medicinal Chemistry Challenges and Opportunities for Bacterial Vaccine Development in the 21<sup>st</sup> Century
Current Pharmaceutical Biotechnology Emergence of Azole Therapy for Cancer Associated Fungal Infections and Their Potential Human Toxicity
The Natural Products Journal Heat Shock Proteins And Neuroprotection
Recent Patents on DNA & Gene Sequences High Contrast and Resolution Labeling of Amyloid Plaques in Tissue Sections from APP-PS1 Mice and Humans with Alzheimer’s Disease with the Zinc Chelator HQ-O: Practical and Theoretical Considerations
Current Alzheimer Research Glycodendrimers as Anti-Adhesion Drugs Against Type 1 Fimbriated E. coli Uropathogenic Infections
Mini-Reviews in Medicinal Chemistry Personalized Nanoparticles for Cancer Therapy: A Call for Greater Precision
Anti-Cancer Agents in Medicinal Chemistry Beyond Oncolytic Virotherapy: Replication-Competent Retrovirus Vectors for Selective and Stable Transduction of Tumors
Current Gene Therapy Ectonucleotidases and Nucleotide/Nucleoside Transporters as Pharmacological Targets for Neurological Disorders
CNS & Neurological Disorders - Drug Targets Recent Trends in Antifungal Agents: A Reference to Formulation, Characterization and Applications
Drug Delivery Letters