Abstract
Separation of 5-(dimethylamino) naphthalene-1-sulphonyl (DNS-) derivatives of some amino acids (alanine, leucine, valine, phenylalanine) D- and L-enantiomers with high-performance thin layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) both with β-cyclodextrin as the component of the mobile phase is presented. The separation mechanism in the PPEC technique involves two effects (partition and electrophoresis) whereas HPTLC system employs only one (partition).
The separation process in both modes was carried out with the HPTLC RP-18 W plates. Influence of variables such as: β-cyclodextrin concentration in the mobile phase and composition of the mobile phase on the migration distance of the solute zones is investigated.
Although HPTLC and PPEC systems enable to separate D- and L-DNS- amino acids the electrophoretic effect in PPECsystem is responsible for different separation selectivity in the latter one in comparison to that in first one. The retention of L-DNS- amino acids is stronger regarding their D-antipodes with both separation techniques. The repeatability of the results from separation of DNS-Ala enantiomers with PPEC and HPTLC technique is compared. Slightly worse standard deviation and RSD values for more retained enantiomer, researched with PPEC, are characteristics of this electromigrational technique.
In addition a new mode of β-cyclodextrin zone determination on the chromatographic plate is presented.
Keywords: β-cyclodextrin, chiral mobile phase, enantiomer separation, DNS-amino acids, HPTLC, PPEC.
Current Analytical Chemistry
Title:β-Cyclodextrin as the Mobile Phase Component for Separation of Some DNS-Amino Acid Enantiomers with HPTLC and PPEC
Volume: 11 Issue: 1
Author(s): Beata Polak and Pawel Garbacz
Affiliation:
Keywords: β-cyclodextrin, chiral mobile phase, enantiomer separation, DNS-amino acids, HPTLC, PPEC.
Abstract: Separation of 5-(dimethylamino) naphthalene-1-sulphonyl (DNS-) derivatives of some amino acids (alanine, leucine, valine, phenylalanine) D- and L-enantiomers with high-performance thin layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) both with β-cyclodextrin as the component of the mobile phase is presented. The separation mechanism in the PPEC technique involves two effects (partition and electrophoresis) whereas HPTLC system employs only one (partition).
The separation process in both modes was carried out with the HPTLC RP-18 W plates. Influence of variables such as: β-cyclodextrin concentration in the mobile phase and composition of the mobile phase on the migration distance of the solute zones is investigated.
Although HPTLC and PPEC systems enable to separate D- and L-DNS- amino acids the electrophoretic effect in PPECsystem is responsible for different separation selectivity in the latter one in comparison to that in first one. The retention of L-DNS- amino acids is stronger regarding their D-antipodes with both separation techniques. The repeatability of the results from separation of DNS-Ala enantiomers with PPEC and HPTLC technique is compared. Slightly worse standard deviation and RSD values for more retained enantiomer, researched with PPEC, are characteristics of this electromigrational technique.
In addition a new mode of β-cyclodextrin zone determination on the chromatographic plate is presented.
Export Options
About this article
Cite this article as:
Polak Beata and Garbacz Pawel, β-Cyclodextrin as the Mobile Phase Component for Separation of Some DNS-Amino Acid Enantiomers with HPTLC and PPEC, Current Analytical Chemistry 2015; 11 (1) . https://dx.doi.org/10.2174/1573411010666141127211110
DOI https://dx.doi.org/10.2174/1573411010666141127211110 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Nanoparticle Drug Delivery Systems: Recent Patents and Applications in Nanomedicine
Recent Patents on Nanomedicine Editorial (Hot Topic: Anti-Inflammatory Drugs and the Heart Special Thematic)
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Assortment of Human Amniotic Membrane and Curcumin: a Potential Therapeutic Strategy for Burn Wound Healing
Current Drug Therapy Scientific Prediction and Prophetic Patenting in Drug Discovery
Recent Patents on CNS Drug Discovery (Discontinued) Abatacept and Glomerular Diseases: The Open Road for the Second Signal as a New Target is Settled Down
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Recombinant Immunotoxins for the Treatment of Chemoresistant Hematologic Malignancies
Current Pharmaceutical Design Cholinergic System and Neuroinflammation: Implication in Multiple Sclerosis
Central Nervous System Agents in Medicinal Chemistry The Mechanism of Mucus Production in Bronchial Asthma
Current Medicinal Chemistry Tumor Stroma as a Target in Cancer
Current Cancer Drug Targets Dendritic Cell-Based Immune Therapy in Liver Diseases
Current Immunology Reviews (Discontinued) New Synthetic Methods to 2-Pyridone Rings
Current Organic Chemistry Effect of DNA Repair Deficiencies on the Cytotoxicity of Drugs Used in Cancer Therapy - A Review
Current Medicinal Chemistry Lactoferrin: A Biologically Active Molecule for Bone Regeneration
Current Medicinal Chemistry Apoptosis Induction by Thalidomide: Critical for Limb Teratogenicity but Therapeutic Potential in Idiopathic Pulmonary Fibrosis?
Current Molecular Pharmacology Controlled Release Inhalable Polymeric Microspheres for Treatment of Pulmonary Arterial Hypertension
Current Pharmaceutical Design About the Guest Editors
Recent Patents on Inflammation & Allergy Drug Discovery An In Silico Appraisal of Azoic and Disulphide Derivatives for Anticancer Activity Against HPV E6 Oncoprotein to Medicate Cervical Cancer
Combinatorial Chemistry & High Throughput Screening Patent Selections:
Recent Patents on Inflammation & Allergy Drug Discovery Quantitative Structure-Activity Relationship (QSAR) Analysis to Predict Drug-Drug Interactions of ABC Transporter ABCG2
Mini-Reviews in Medicinal Chemistry The Relationship Between HIV Infection and Cardiovascular Disease
Current Cardiology Reviews