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Current Analytical Chemistry


ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Detection of Phenolic Compounds by Tyrosinase Modified Clark Type Electrode

Author(s): Margarita Stoytcheva, Roumen Zlatev, Mary Triny Beleno and Gisela Montero

Volume 11, Issue 1, 2015

Page: [50 - 55] Pages: 6

DOI: 10.2174/1573411010666141119220515

Price: $65


In this work is suggested a Clark type electrode with disposable enzyme membrane for phenols determination. This approach allows avoiding the electrode fouling by recording the oxygen reduction current, and obtaining a stable and reproducible sensor response. An appropriate method for enzyme membrane fabrication by cellulose acetate filters coating with tyrosinase through the use of biocompatible hydrogels was proposed and tested. The sensitivity of the catechol determination, as an example, was found to be 0.89 µA mmol-1 L. The detection limit equaled 1 µmol L-1. The achieved RSD was 1.22%.

Keywords: Clark type electrode, tyrosinase, catechol, disposable membrane, hydrogel.

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