Abstract
The pharmaceutical industry as well as European and US governing agencies have indicated the need for more accurate, high resolution, characterization of complex drug materials, nanomedicines, to facilitate their development and eventual approval. In particular, accurately measuring the size, zeta-potential, and concentration of nanomedicines is desired. Herein we demonstrate the comprehensive and high resolution analysis capabilities of tunable resistive pulse sensing (TRPS) on the most widely approved nanomedicines to-date, liposomal particles. The number-based size distribution, concentration and volume fraction of liposomes formed by extrusion through a 100 nm or 200 nm Nucleopore filter membrane are shown as well as how freeze-thaw aggregation changes individual liposomes and the overall size distribution. In addition, the simultaneous size and zeta-potential analysis capabilities of TRPS is used to characterize the homogeneity and difference between liposomes made with and without the addition of PEGylated phospholipids.
Keywords: Coulter counter, particle characterization, pore sensor, TRPS, qNano.
Current Drug Delivery
Title:High Resolution Particle Characterization to Expedite Development and Regulatory Acceptance of Nanomedicines
Volume: 12 Issue: 1
Author(s): Darby Kozak, Murray Broom and Robert Vogel
Affiliation:
Keywords: Coulter counter, particle characterization, pore sensor, TRPS, qNano.
Abstract: The pharmaceutical industry as well as European and US governing agencies have indicated the need for more accurate, high resolution, characterization of complex drug materials, nanomedicines, to facilitate their development and eventual approval. In particular, accurately measuring the size, zeta-potential, and concentration of nanomedicines is desired. Herein we demonstrate the comprehensive and high resolution analysis capabilities of tunable resistive pulse sensing (TRPS) on the most widely approved nanomedicines to-date, liposomal particles. The number-based size distribution, concentration and volume fraction of liposomes formed by extrusion through a 100 nm or 200 nm Nucleopore filter membrane are shown as well as how freeze-thaw aggregation changes individual liposomes and the overall size distribution. In addition, the simultaneous size and zeta-potential analysis capabilities of TRPS is used to characterize the homogeneity and difference between liposomes made with and without the addition of PEGylated phospholipids.
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Cite this article as:
Kozak Darby, Broom Murray and Vogel Robert, High Resolution Particle Characterization to Expedite Development and Regulatory Acceptance of Nanomedicines, Current Drug Delivery 2015; 12 (1) . https://dx.doi.org/10.2174/1567201811666140922110647
DOI https://dx.doi.org/10.2174/1567201811666140922110647 |
Print ISSN 1567-2018 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5704 |
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