Current Approaches to Glycoprotein Analysis

Title:Current Approaches to Glycoprotein Analysis

Volume: 21 Issue: 10

Author(s): Andrew K. Hagan, Maomao Wang and Li Liu

Affiliation:

Keywords: Analytical techniques, chemical derivatization, glycosylation, HPLC, mass spectrometry, proteases, therapeutic glycoproteins.

Abstract: Glycoproteins are becoming increasingly relevant in therapeutics, including tissue plasminogen activator for the treatment of myocardial infarction and strokes, erythropoietin for anemia and various monoclonal antibody–based treatments for cancer. Protein N- and O-glycosylation is perhaps the most crucial and immensely complex posttranslational modification that proteins undergo, and its characterization presents a major challenge. This review will discuss current techniques for the characterization of glycoproteins, with a focus on therapeutic glycoproteins where available. The crucial analytical techniques, such as high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and mass spectrometry (MS) will be described, alongside the necessary chemical labeling methods for sensitive detection. The well-established chemical and enzymatic methods for oligosaccharide release from proteins will be discussed, as will more modern methods based on exhaustive protein hydrolysis with non-specific proteases.

Cite this article as:

Hagan K. Andrew, Wang Maomao and Liu Li, Current Approaches to Glycoprotein Analysis, Protein & Peptide Letters 2014; 21 (10) . https://dx.doi.org/10.2174/0929866521666140626095752