Abstract
Enzyme-catalyzed mono- and oligosaccharide production is a prime example of the potential of multi-enzyme reaction systems, where the regio- and reaction selectivity of these biocatalysts allows the implementation of complex reaction cascades in one vessel where alternative approaches would need lengthy multi-vessel reaction sequences and protection group chemistry. However, this introduces as a new challenge the question of how such multi-enzyme systems can efficiently be assembled in the face of increasing system complexity, which is increasingly addressed by setting up pathways to saccharide production in perforated or intact cells. Put differently, the assembly of such pathways becomes more and more a biosystems engineering activity which needs to take into consideration the need to fine-tune system composition and interactions with the cellular background. This is precisely the domain of synthetic biology, which can be classified as a biosystems engineering activity developing around our increasing capacity to assemble large sets of genes de novo. Here, we argue that biocatalyst construction in saccharide synthesis can broadly benefit from the tools that are being developed in the domain of synthetic biology.
Keywords: Metabolic engineering, mono- and oligosaccharide production, synthetic biology, whole cell systems.
Graphical Abstract
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
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