Partitioning studies of invertase are carried out by incorporating different nanoparticles at varied concentrations (0-2 %, w/w) employing polymer/polymer (PEG-3350/dextran-T70) and polymer/salt (PEG-3350/magnesium sulphate) phase systems. The partition coefficient of total protein (KTP) is found to decrease in case of PEG-3350/dextran-T70 system with an increase in concentration of nanoparticles. However, the extent of decrease varied depending on the nanoparticle. Significant changes are not observed in partition coefficient of invertase (KINV). In case of PEG- 3350/magnesium sulphate systems, gradual increase in KTP and KINV values is observed with an increase in concentration of nanoparticles. The partition coefficient varied (KTP from 0.06 to 0.25 and KINV from 0.01 to 0.04) depending on the nanoparticles. Highest enrichment of enzyme is observed in case of PEG-3350/magnesium sulphate system with gold nanoparticles (32.64 U/mg specific activity with 9 fold enrichment and enzyme activity recovery of 92%) among all the systems studied.
Keywords: Aqueous two phase extraction, invertase, partition coefficient, nanoparticles, purification, recovery.