Abstract
The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.
Keywords: Cancer, high content analysis, phenotypic screening, RNAi screening, statistical analysis.
Combinatorial Chemistry & High Throughput Screening
Title:Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute
Volume: 17 Issue: 4
Author(s): Emma J. Shanks
Affiliation:
Keywords: Cancer, high content analysis, phenotypic screening, RNAi screening, statistical analysis.
Abstract: The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.
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Cite this article as:
Shanks J. Emma, Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute, Combinatorial Chemistry & High Throughput Screening 2014; 17 (4) . https://dx.doi.org/10.2174/1386207317666140323133249
DOI https://dx.doi.org/10.2174/1386207317666140323133249 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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