Abstract
Antibodies have emerged as powerful therapeutics effective for treating a number of human conditions and diseases. While early successes utilized small animals to generate therapeutic antibodies, human antibodies are now preferred in order to limit anti-antibody immune responses. Antibodies with human amino acid sequences can be generated in a number of ways, such as humanizing antibodies from other species or expressing human antibodies in transgenic animals. This review focuses on methods for obtaining antibodies directly from human B cells. These methods use both antigen exposed and non-exposed (“naïve”) humans as B cell sources, and apply various technologies to isolate desired antibodies; including cell line generation, single cell isolation, display technologies, and B cell library generation.
Keywords: Antibody engineering, antibody libraries, display technologies, high-throughput screening, human monoclonal antibodies, human B cell.
Current Drug Discovery Technologies
Title:Different Approaches for Obtaining Antibodies from Human B Cells
Volume: 11 Issue: 1
Author(s): Marcus R. Duvall and Ryan N. Fiorini
Affiliation:
Keywords: Antibody engineering, antibody libraries, display technologies, high-throughput screening, human monoclonal antibodies, human B cell.
Abstract: Antibodies have emerged as powerful therapeutics effective for treating a number of human conditions and diseases. While early successes utilized small animals to generate therapeutic antibodies, human antibodies are now preferred in order to limit anti-antibody immune responses. Antibodies with human amino acid sequences can be generated in a number of ways, such as humanizing antibodies from other species or expressing human antibodies in transgenic animals. This review focuses on methods for obtaining antibodies directly from human B cells. These methods use both antigen exposed and non-exposed (“naïve”) humans as B cell sources, and apply various technologies to isolate desired antibodies; including cell line generation, single cell isolation, display technologies, and B cell library generation.
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Cite this article as:
Duvall R. Marcus and Fiorini N. Ryan, Different Approaches for Obtaining Antibodies from Human B Cells, Current Drug Discovery Technologies 2014; 11 (1) . https://dx.doi.org/10.2174/1570163811666140121154649
DOI https://dx.doi.org/10.2174/1570163811666140121154649 |
Print ISSN 1570-1638 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6220 |
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