Abstract
Aicardi Goutieres Syndrome (AGS) is characterized by mutations occurring in genes encoding RNAses. AGS mutations silence intracellular RNases resulting in an intracellular overload of short RNAs arresting the physiological production of microRNA required for brain development. MiR-219 is typically down-regulated in Aicardi Goutieres Syn-drome (AGS). The goal of this study is to investigate miR-219 role in protecting astrocytes co-cultured with AGS-mutated lymphocytes. These lymphocytes display neurotoxic properties due to the presence of AGS-mutation and to their activa-tion by interpheron-alpha (IFN). Obtained results provide the evidence that astrocytes transfected with microRNA-219 are protected from the neurotoxic action of AGS lymphocytes activated by IFN-alpha. In particular, the miR-219 transfection increased astrocyte viability, growth, and differentiation while decreasing cell necrosis and apoptosis. Thus, microRNA-219 transfection is a valuable strategy in order to confer resistance to astrocytes towards lymphocyte-induced neurotoxici-ty especially in the presence of IFN-alpha, whose levels are typically increased in the cerebrospinal fluid of AGS patients.
Keywords: Aicardi Goutieres Syndrome, interferon-alpha, lymphocytes, microRNA, miR-219 transfection, neurotoxicity.
MicroRNA
Title:Prevention of Lymphocyte Neurotoxic Effects by microRNA Delivery
Volume: 2 Issue: 3
Author(s): Alessandra Pulliero, Barbara Marengo, Daniela Fenoglio, Alessia Parodi, Cristina Cereda, Cinzia Domenicotti, Simona Orcesi, Jessica Galli, Ivana Olivieri, Gilberto Filaci, Umberto Balottin, Elisa Fazzi and Alberto Izzotti
Affiliation:
Keywords: Aicardi Goutieres Syndrome, interferon-alpha, lymphocytes, microRNA, miR-219 transfection, neurotoxicity.
Abstract: Aicardi Goutieres Syndrome (AGS) is characterized by mutations occurring in genes encoding RNAses. AGS mutations silence intracellular RNases resulting in an intracellular overload of short RNAs arresting the physiological production of microRNA required for brain development. MiR-219 is typically down-regulated in Aicardi Goutieres Syn-drome (AGS). The goal of this study is to investigate miR-219 role in protecting astrocytes co-cultured with AGS-mutated lymphocytes. These lymphocytes display neurotoxic properties due to the presence of AGS-mutation and to their activa-tion by interpheron-alpha (IFN). Obtained results provide the evidence that astrocytes transfected with microRNA-219 are protected from the neurotoxic action of AGS lymphocytes activated by IFN-alpha. In particular, the miR-219 transfection increased astrocyte viability, growth, and differentiation while decreasing cell necrosis and apoptosis. Thus, microRNA-219 transfection is a valuable strategy in order to confer resistance to astrocytes towards lymphocyte-induced neurotoxici-ty especially in the presence of IFN-alpha, whose levels are typically increased in the cerebrospinal fluid of AGS patients.
Export Options
About this article
Cite this article as:
Pulliero Alessandra, Marengo Barbara, Fenoglio Daniela, Parodi Alessia, Cereda Cristina, Domenicotti Cinzia, Orcesi Simona, Galli Jessica, Olivieri Ivana, Filaci Gilberto, Balottin Umberto, Fazzi Elisa and Izzotti Alberto, Prevention of Lymphocyte Neurotoxic Effects by microRNA Delivery, MicroRNA 2013; 2 (3) . https://dx.doi.org/10.2174/22115366113029990010
DOI https://dx.doi.org/10.2174/22115366113029990010 |
Print ISSN 2211-5366 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-5374 |
Call for Papers in Thematic Issues
Modulatory Roles of Non-coding RNAs in cancer therapy
This thematic issue aims to elucidate the most recent research on ncRNAs in CRCs and discuss the importance and difficulties that may arise in applying this tool on a regular clinical basis.read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Anti-inflammatory and Immune Therapy for Alzheimers Disease: Current Status and Future Directions
Current Neuropharmacology Anti-Vascular Endothelial Growth Factor Treatment in Retinal Vein Occlusions
Current Drug Therapy Stem Cell Regenerative Potential Combined with Nanotechnology and Tissue Engineering for Myocardial Regeneration
Current Stem Cell Research & Therapy Microbial Exo-Polysaccharides for Biomedical Applications
Mini-Reviews in Medicinal Chemistry The Animal Models of Dementia and Alzheimer’s Disease for Pre-Clinical Testing and Clinical Translation
Current Alzheimer Research Nocistatin: Milestone of One Decade of Research
Current Pharmaceutical Design On Some Physiological Aspects of Ethanol Repercussion on Neural and Cardiorenal Functions
Central Nervous System Agents in Medicinal Chemistry Nonviral Approach for Targeted Nucleic Acid Delivery
Current Medicinal Chemistry Perspectives on the Development of Antioxidant Antiepileptogenic Agents
Mini-Reviews in Medicinal Chemistry Traditional and Alternative Therapies for Refractory Angina
Current Pharmaceutical Design Cholinergic Treatment of Traumatic Brain Injury
Current Drug Therapy Functional and Molecular Ultrasound Imaging: Concepts and Contrast Agents
Current Medicinal Chemistry COVID-19 Infection in Pregnancy: A Review
Infectious Disorders - Drug Targets Substance P: Structure, Function, and Therapeutics
Current Topics in Medicinal Chemistry Advanced Techniques for Imaging the Human Spinal Cord: Review of Literature
Current Medical Imaging Current Development in Encapsulated Cell Therapy for Degenerative Retinopathies
Current Tissue Engineering (Discontinued) Genetic Determinants of Amyotrophic Lateral Sclerosis as Therapeutic Targets
CNS & Neurological Disorders - Drug Targets Hypovitaminosis D in Patients with Ankylosing Spondylitis: Frequency and Consequences
Current Rheumatology Reviews Recent Advances in the Treatment of Neurogenic Erectile Dysfunction
Recent Patents on CNS Drug Discovery (Discontinued) Idiopathic Transverse Myelitis and Neuromyelitis Optica: Clinical Profiles,Pathophysiology and Therapeutic Choices
Current Neuropharmacology