Abstract
A nonenzymatic biosensor for hydrogen peroxide (H2O2) is developed based on a glassy carbon electrode loaded with layered nano-structured perovskite-type oxide LaNiTiO3 by sol-gel method. Characterizations of as-synthesized LaNiTiO3 are conducted using powder X-ray diffraction, transmission electron microscopy, scanning electron microscopy and low temperature N2 adsorption and desorption isotherms techniques. Electrochemical properties of the fabricated biosensor are studied by cyclic voltammetry and currenttime curves. Experimental results show the electrochemical biosensor has high activity to the oxidation of H2O2 with a fast response time of ~ 2 s in 0.1 M NaOH solution. A wide linearity is obtained from 0.05 μM to 10.0 m with a low detection limit of 0.01 μM (S/N = 3) and a high sensitivity of 854 μA mM-1 cm-2 under optimal conditions: the content of LaNiTiO3 modifier (5.0 μL, 0.5 mg/mL) and applied potential (0.55 V). The biosensor also exhibits good stability, reproducibility and anti-interference ability, suggesting potential practical application of LaNiTiO3 in biological analysis and environmental detection.
Keywords: Biosensor, detection, electrochemistry, hydrogen peroxide, nanomaterial, perovskite.
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