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Current Pharmaceutical Design


ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

SNAP-tag as a Tool for Surface Immobilization

Author(s): Sinem Engin, Dagmar Fichtner, Doris Wedlich and Ljiljana Fruk

Volume 19, Issue 30, 2013

Page: [5443 - 5448] Pages: 6

DOI: 10.2174/1381612811319300015

Price: $65


SNAP-tag technology has been an important tool for protein study for more than a decade and in the meanwhile has found a number of applications beyond the field of molecular biology and protein purification. Based on covalent interaction of SNAP-tag, 20 kDA mutant of DNA repair protein and benzylguanine, it enables irreversible and controllable protein modification. In this mini review, recent developments in the use of SNAP-tag for the design of protein arrays and nanoparticle biofunctionalization are presented and discussed. A short overview of other applications that paved the way to surface modifications is also given with emphasis on fluorescent labeling through the use of SNAP-tag fusion proteins. Finally, the future of the SNAP-tag methodology for surface patterning and 3D structural scaffolding is addressed.

Keywords: SNAP-tag, fusion proteins, benzylguanine, micro-contact printing, protein-nanoparticle conjugation, DNA directed immobilization, DNA origami.

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