Abstract
Glutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays a key role in controlling cellular thiol redox system, maintaining the immune and detoxification system. The determination of GSH levels in tissue is important to estimate endogenous defenses against oxidative stress. In our study, the multi-walled carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used to determine the levels of GSH in trichloroacetic acid (TCA)-treated or untreated samples of rat plasma. It was found that the deproteinization of samples with TCA improved the electrochemical detection of GSH particularly in plasma. The oxidation of GSH was measured by using differential pulse voltammetry (DPV) method in combination with MWCNT-SPE (n=3), and the detection limit of GSH was found to be 0.47 µM (S/N=3). The GSH levels in plasma samples were also measured spectrophotometrically in order to compare the effectiveness of electrochemical method and we obtained a high correlation between the two methods (R2=0.976).
Keywords: Differential pulse voltammetry, glutathione, multi-walled carbon nanotubes, plasma samples, screen-printed electrodes, spectrophotometry.
Combinatorial Chemistry & High Throughput Screening
Title:Electrochemical Determination of Glutathione in Plasma at Carbon Nanotubes Based Screen Printed Electrodes
Volume: 16 Issue: 9
Author(s): Ezgi Turunc, Hakan Karadeniz, Guliz Armagan, Arzum Erdem and Ayfer Yalcin
Affiliation:
Keywords: Differential pulse voltammetry, glutathione, multi-walled carbon nanotubes, plasma samples, screen-printed electrodes, spectrophotometry.
Abstract: Glutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays a key role in controlling cellular thiol redox system, maintaining the immune and detoxification system. The determination of GSH levels in tissue is important to estimate endogenous defenses against oxidative stress. In our study, the multi-walled carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used to determine the levels of GSH in trichloroacetic acid (TCA)-treated or untreated samples of rat plasma. It was found that the deproteinization of samples with TCA improved the electrochemical detection of GSH particularly in plasma. The oxidation of GSH was measured by using differential pulse voltammetry (DPV) method in combination with MWCNT-SPE (n=3), and the detection limit of GSH was found to be 0.47 µM (S/N=3). The GSH levels in plasma samples were also measured spectrophotometrically in order to compare the effectiveness of electrochemical method and we obtained a high correlation between the two methods (R2=0.976).
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Cite this article as:
Turunc Ezgi, Karadeniz Hakan, Armagan Guliz, Erdem Arzum and Yalcin Ayfer, Electrochemical Determination of Glutathione in Plasma at Carbon Nanotubes Based Screen Printed Electrodes, Combinatorial Chemistry & High Throughput Screening 2013; 16 (9) . https://dx.doi.org/10.2174/13862073113169990040
DOI https://dx.doi.org/10.2174/13862073113169990040 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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