Abstract
Serotonin is a monoamine neurotransmitter and biochemically derived from tryptophan. The present paper examines the in vitro antioxidant and radical scavenging capacity of serotonin using different in vitro methodologies. For the determination of antioxidant activity of serotonin, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH-) scavenging, 2,2´- azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination by Fe3+-Fe2+ transformation method, superoxide anion radical scavenging by riboflavin-methionine-illuminate system, H2O2 scavenging and ferrous ions (Fe2+) chelating activities of serotonin were performed. Also, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and trolox, a water-soluble analog of α-tocopherol, were used as the reference antioxidant radical scavenger compounds. Serotonin completely inhibited lipid peroxidation of a linoleic acid emulsion at 15 µg/mL concentration. On the other hand, the above mentioned standard antioxidants displayed inhibition of 92.2, 99.6, 84.6 and 95.6% on peroxidation of linoleic acid emulsion, respectively at the 45 µg/mL concentration. In addition, serotonin was effective in DPPH· scavenging, ABTS•+ radical scavenging, superoxide anion radical scavenging, H2O2 scavenging, ferric ions (Fe3+) reducing power and metal chelating on ferrous ions (Fe2+) activities. Also, those various antioxidant activities were compared to BHA, BHT α-tocopherol and trolox as references antioxidant compounds.
Keywords: Serotonin, antioxidant activity, metal chelating, reducing power, radical scavenging.
Current Bioactive Compounds
Title:Radical Scavenging and Antioxidant Capacity of Serotonin
Volume: 9 Issue: 2
Author(s): S. Beyza Ozturk Sarikaya and Ilhami Gulcin
Affiliation:
Keywords: Serotonin, antioxidant activity, metal chelating, reducing power, radical scavenging.
Abstract: Serotonin is a monoamine neurotransmitter and biochemically derived from tryptophan. The present paper examines the in vitro antioxidant and radical scavenging capacity of serotonin using different in vitro methodologies. For the determination of antioxidant activity of serotonin, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH-) scavenging, 2,2´- azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination by Fe3+-Fe2+ transformation method, superoxide anion radical scavenging by riboflavin-methionine-illuminate system, H2O2 scavenging and ferrous ions (Fe2+) chelating activities of serotonin were performed. Also, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and trolox, a water-soluble analog of α-tocopherol, were used as the reference antioxidant radical scavenger compounds. Serotonin completely inhibited lipid peroxidation of a linoleic acid emulsion at 15 µg/mL concentration. On the other hand, the above mentioned standard antioxidants displayed inhibition of 92.2, 99.6, 84.6 and 95.6% on peroxidation of linoleic acid emulsion, respectively at the 45 µg/mL concentration. In addition, serotonin was effective in DPPH· scavenging, ABTS•+ radical scavenging, superoxide anion radical scavenging, H2O2 scavenging, ferric ions (Fe3+) reducing power and metal chelating on ferrous ions (Fe2+) activities. Also, those various antioxidant activities were compared to BHA, BHT α-tocopherol and trolox as references antioxidant compounds.
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Cite this article as:
Sarikaya Beyza Ozturk S. and Gulcin Ilhami, Radical Scavenging and Antioxidant Capacity of Serotonin, Current Bioactive Compounds 2013; 9 (2) . https://dx.doi.org/10.2174/22115528112019990006
DOI https://dx.doi.org/10.2174/22115528112019990006 |
Print ISSN 1573-4072 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6646 |
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Antioxidants have captured significant interest for decades due to their potential role in preventing chronic diseases. These molecules combat free radicals and unstable molecules that damage cells and contribute to the development of conditions like metabolic diseases, heart disease, cancer, and neurodegenerative diseases. This thematic issue will explore the cutting-edge ...read more
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The Special Issue "Bioactive Compounds: Discovery, Design, Development, and Target Identification" aims to bring together innovative research and critical reviews on bioactive molecules that show therapeutic potential across diverse diseases. Discovery of novel bioactive compounds will focus on uncovering new molecules from natural or synthetic sources, expanding the library of ...read more

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