Abstract
There are a number of stigmas attached to the development of antitumor drugs such as their safe and efficient delivery into target cells or tissues. The one such case is that of macromolecules, such as nucleic acids where it poses severe limits. From this point of view, electro-pulsation proves to be a promising method for cancer-associated gene therapy. It involves the direct application of electric pulses on cells or tissues which leads to a transient permeabilization of their plasma membrane allowing efficient in vitro and in vivo delivery of exogenous molecules. The present review probes the electrotransfer of nucleic acids, the nature of nucleic acids (plasmid DNA, mRNA, siRNA, LNA...) which can be electrotransferred and the mechanism of their electrotransfer.
Keywords: Electroporation, gene delivery, RNAi, tumor, Nucleic Acids, Electro-transfer, Antitumor Drugs, macromolecules, Nucleic Acid, DNA, LNA, SiRNA, Plasmid
Current Drug Metabolism
Title:Nucleic Acids Electro-transfer: From Bench to Bedside
Volume: 14 Issue: 3
Author(s): Sophie Chabot, Christelle Rosazza, Muriel Golzio, Andreas Zumbusch, Justin Teissie and Marie-Pierre Rols
Affiliation:
Keywords: Electroporation, gene delivery, RNAi, tumor, Nucleic Acids, Electro-transfer, Antitumor Drugs, macromolecules, Nucleic Acid, DNA, LNA, SiRNA, Plasmid
Abstract: There are a number of stigmas attached to the development of antitumor drugs such as their safe and efficient delivery into target cells or tissues. The one such case is that of macromolecules, such as nucleic acids where it poses severe limits. From this point of view, electro-pulsation proves to be a promising method for cancer-associated gene therapy. It involves the direct application of electric pulses on cells or tissues which leads to a transient permeabilization of their plasma membrane allowing efficient in vitro and in vivo delivery of exogenous molecules. The present review probes the electrotransfer of nucleic acids, the nature of nucleic acids (plasmid DNA, mRNA, siRNA, LNA...) which can be electrotransferred and the mechanism of their electrotransfer.
Export Options
About this article
Cite this article as:
Chabot Sophie, Rosazza Christelle, Golzio Muriel, Zumbusch Andreas, Teissie Justin and Rols Marie-Pierre, Nucleic Acids Electro-transfer: From Bench to Bedside, Current Drug Metabolism 2013; 14 (3) . https://dx.doi.org/10.2174/1389200211314030005
DOI https://dx.doi.org/10.2174/1389200211314030005 |
Print ISSN 1389-2002 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5453 |
Call for Papers in Thematic Issues
Interaction between drugs and endocrine diseases
The introduction of highly active antiretroviral therapy accelerated studies and our understanding on the interaction between pharmacological therapies and endocrine diseases. Drugs can precipitate endocrine via different mechanisms, including direct alteration of hormone production and secretion, dysregulation of hormonal axis, effects on hormonal transport, receptor-binding, and cellular signalling. Common drug-induced ...read more
Tissue Distribution and Metabolism of Micro- and Nanoparticles and Medical Implants
With the continuous advancement of modern science and engineering, numerous functional materials and active molecules have been developed and utilized in various industrial, medical, and food applications. Many of these can enter the body, either actively or passively, and have significant and intricate impacts on human health. For example, biomaterials ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Deregulation of PI3K/Akt/mTOR Signaling Pathways by Isoflavones and its Implication in Cancer Treatment
Anti-Cancer Agents in Medicinal Chemistry Concentration-Dependent Bimodal Effect of Specific 18 kDa Translocator Protein (TSPO) Ligands on Cell Death Processes Induced by Ammonium Chloride: Potential Implications for Neuropathological Effects Due to Hyperammonemia
CNS & Neurological Disorders - Drug Targets Berberine Exhibits Antitumor Effects in Human Ovarian Cancer Cells
Anti-Cancer Agents in Medicinal Chemistry Regulation of Cellular Signal Transduction Pathways by the Extracellular Calcium-Sensing Receptor
Current Pharmaceutical Biotechnology Ubiquitin-Protein Ligases - Novel Therapeutic Targets?
Current Protein & Peptide Science Cellular and Physiological Effects of Soy Flavonoids
Mini-Reviews in Medicinal Chemistry Drug Metabolism and Transport Under Hypoxia
Current Drug Metabolism Stat3 Orchestrates Tumor Development and Progression: The Achilles Heel of Head and Neck Cancers?
Current Cancer Drug Targets COXIBs, CINODs and H2S-Releasing NSAIDs: Current Perspectives in the Development of Safer Non Steroidal Anti-Inflammatory Drugs
Current Medicinal Chemistry Pleiotropic Beneficial Effects of Epigallocatechin Gallate, Quercetin and Delphinidin on Cardiovascular Diseases Associated with Endothelial Dysfunction
Cardiovascular & Hematological Agents in Medicinal Chemistry TGF-β Signaling in Gastrointestinal Cancer Stem Cells
Current Cancer Therapy Reviews Protective Effect of Annurca Apple Extract Against Oxidative Damage in Human Erythrocytes
Current Nutrition & Food Science Functionalized magnetic nanoparticles for biomedical applications
Current Pharmaceutical Design Natural Products as Anticancer Agents
Current Drug Targets Betulinic Acid as a Potent and Complex Antitumor Phytochemical: A Minireview
Anti-Cancer Agents in Medicinal Chemistry Preventative and Therapeutic Potential of Lipopolysaccharide Derived from Edible Gram-Negative Bacteria to Various Diseases
Current Drug Therapy Editorial [Hot topic: Models and Evolution of Inflammatory Activities (Executive Editor: Giuseppe Scapigliati)]
Current Pharmaceutical Design Biologically Active Phytochemicals: A Brief Appraisal
Current Traditional Medicine Management of Postoperative Nausea and Vomiting in Women Undergoing Major Gynecological Surgery: A Review and Update
Current Drug Therapy Therapeutic Potential of Nitrate Esters of Commonly Used Drugs
Current Topics in Medicinal Chemistry