A micro/nanostructured composite electrode was constructed by introducing various portions of aluminum titanate nanopowder and gold nanoparticle into glassy carbon paste electrode and used for glutathione (GSH) detection. After the optimization of experimental parameters, the analytical characteristics of the developed system were investigated. Two linear ranges between 5-50 μM and 100-750 μM with the equation y = 0.0028x+0.0102 and y= 4e-5x+ 0.227 with the correlation coefficients of R2= 0.9943 and R2= 0.9878 were obtained.
Relative standard deviation value was calculated for 250 μM GSH (n=6) and found as 6.66 %. The developed sensor was also applied for the detection of GSH in wine sample and human urine sample by using standard addition method. Data obtained from the sample application part showed that this novel nanocomposite response was not affected by the sample matrix and the nature without needing any laborious sample pretreatment procedures.
Keywords: Aluminum titanate nanopowder, cyclic voltammetry, electrochemical sensor, glassy carbon paste electrode, gold nanoparticle, GSH, human urine sample, micro/nanostructured composite electrode, nanocomposite, wine sample