Microarray-Based Identification of New Targets for Specific Therapies in Pediatric Leukemia

Monique   L.   den Boer; Rob      Pieters

doi:10.2174/138945007780830755

Abstract

The efficacy of current treatment protocols for childhood cancer is mainly based on empirical studies by adding drugs, changing drug dosages and changing drug combinations. In pediatric acute lymphoblastic leukemia (ALL), this approach has resulted into ∼80% 5-year disease-free survival whereas less favorable results have yet been obtained for acute myeloid leukemia (AML), i.e. ∼50%, and other types of tumors, e.g. ∼60% for medulloblastoma. A further optimization of therapy results requires more insights into the molecular biology of tumor cells, including genetic defects and aberrant expression of genes. This knowledge is needed to rationally develop more specific therapies in which relapse-risk and side-effects of therapy are reduced using targeted drugs. Genome-wide analysis of gene expression levels (mRNA) has revealed many new insights into the biology of leukemic cells. In this review we will discuss the recent progress that has been made in the use of microarrays for identifying new markers and targets for treatment of acute leukemia in children.

Keywords: Gene expression profiling, microarray, classification, drug resistance, outcome, targeted therapy, childhood, acute leukemia