Anti-Oxidative Effect of Curcumin Against Tert-Butylhydroperoxide Induced Oxidative Stress in Human Erythrocytes

Title: Anti-Oxidative Effect of Curcumin Against Tert-Butylhydroperoxide Induced Oxidative Stress in Human Erythrocytes

Volume: 2 Issue: 1

Author(s): Prabhakar Singh and Syed Ibrahim Rizvi

Affiliation:

Keywords: Antioxidant, curcumin, erythrocyte, glutathione, malondialdehyde, oxidative stress, Tert-Butylhydroperoxide, lipid peroxidation, free radical

Abstract: Curcumin (diferuloylmethane, 1, 6-heptadiene-3, 5-5dione-1, 7-bis (4-hydroxy-3-methoxyphenyl)), a constituent of turmeric (curcuma longa), is traditionally used in various diseases. We report the strong anti-oxidative effect of curcumin on markers of oxidative stress in human erythrocytes subjected to in vitro oxidative stress by incubating with tert-butylhydroperoxide (t-BHP). Results show that curcumin protects erythrocyte lipid peroxidation (MDAMalondialdehyde) and decrease of glutathione (GSH) during oxidative stress. The quotient of MDA/GSH is high during stress however presence of curcumin (10-8 M-10-5 M) decreased the index (MDA/GSH) substantiating the antioxidant effect of curcumin in a model cell system. The effect of curcumin is dose-dependent with maximum effect at 10-5M. Since oxidative stress has been implicated in the pathogenesis of many diseases/conditions, a therapeutic role of curcumin is hypothesized.

Cite this article as:

Singh Prabhakar and Ibrahim Rizvi Syed, Anti-Oxidative Effect of Curcumin Against Tert-Butylhydroperoxide Induced Oxidative Stress in Human Erythrocytes, The Natural Products Journal 2012; 2 (1) . https://dx.doi.org/10.2174/2210315511202010069