Generating Recombinant Antibodies for Research, Diagnostics and Therapy Using Phage Display

Andre      Frenzel; David      Frode; Torsten      Meyer; Thomas      Schirrmann; Michael      Hust

Abstract

Since the development of antibody phage display, 20 years ago, this technique has been evolved to a valuable and robust tool for the development of recombinant human antibodies. Today, this technique is widely used for the generation of antibodies for therapy and basic research. Automation processes and decrease of costs will enable the highthroughput design of monoclonal binders against a huge number of different antigens, presenting a valuable tool for proteome research. This review presents an overview on phage display technology and antibody libraries. The benefit of recombinant antibodies for basic research, diagnostic and therapy is shown.

Keywords: phage display, scFv, antibody, proteome research, antibody gene library, monoclonal antibody, human antibody, Humira, antibody genes, Murine antibodies, B-lymphocytes from, IgGs, E. coli, phagemid, bacteriophage M13