Abstract
Arrays of human umbilical cord blood-neural stem cells have been patterned in high density at single cell resolution. Pre-patterns of adhesive molecules, i.e. fibronectin and poly-L-lysine, have been produced on anti-adhesive poly (ethylene) oxide films deposited by plasma-enhanced chemical vapour deposition, which prevents cell adsorption. The structures consisted of adhesive squares and lines with 10μm lateral dimensions, which correspond approximately to the size of one cell nucleus, separated by 10μm anti-adhesive gap. The stem cells cultured on these platforms redistribute their cytoplasm on the permitted areas. Spherical cells were deposited on the square patterns in a single cell mode, while on the lines they spread longitudinally; the extent of elongation being dependent on the specific (fibronectin) or non-specific (poly-L-lysine) attachment biomolecule. The cell patterns were retained up to 12 days, which will be useful for recording statistical data of individual chronic responses to chemical, physical or physiologically relevant stimuli.
Keywords: Micropatterning, single cell, cell adhesion, stem cell, polylysine, fibronectin
Micro and Nanosystems
Title: Single Stem Cell Positioning on Polylysine and Fibronectin Microarrays
Volume: 1 Issue: 1
Author(s): A. Ruiz, M. Zychowicz, L. Buzanska, D. Mehn, C. A. Mills, E. Martinez, S. Coecke, J. Samitier, P. Colpo and F. Rossi
Affiliation:
Keywords: Micropatterning, single cell, cell adhesion, stem cell, polylysine, fibronectin
Abstract: Arrays of human umbilical cord blood-neural stem cells have been patterned in high density at single cell resolution. Pre-patterns of adhesive molecules, i.e. fibronectin and poly-L-lysine, have been produced on anti-adhesive poly (ethylene) oxide films deposited by plasma-enhanced chemical vapour deposition, which prevents cell adsorption. The structures consisted of adhesive squares and lines with 10μm lateral dimensions, which correspond approximately to the size of one cell nucleus, separated by 10μm anti-adhesive gap. The stem cells cultured on these platforms redistribute their cytoplasm on the permitted areas. Spherical cells were deposited on the square patterns in a single cell mode, while on the lines they spread longitudinally; the extent of elongation being dependent on the specific (fibronectin) or non-specific (poly-L-lysine) attachment biomolecule. The cell patterns were retained up to 12 days, which will be useful for recording statistical data of individual chronic responses to chemical, physical or physiologically relevant stimuli.
Export Options
About this article
Cite this article as:
Ruiz A., Zychowicz M., Buzanska L., Mehn D., Mills A. C., Martinez E., Coecke S., Samitier J., Colpo P. and Rossi F., Single Stem Cell Positioning on Polylysine and Fibronectin Microarrays, Micro and Nanosystems 2009; 1 (1) . https://dx.doi.org/10.2174/1876402910901010050
DOI https://dx.doi.org/10.2174/1876402910901010050 |
Print ISSN 1876-4029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1876-4037 |
Call for Papers in Thematic Issues
Quantum AI and Quantum Computing for Data Analytics
Quantum Computing has emerged as a major research interest in recent decades. Researchers and Industries are showing their research interest in Quantum Computing in many aspects like Quantum Machine Learning, Quantum AI, Quantum optimization, and many more. The advancement in quantum computing in the recent era is called the quantum ...read more
Recent Advances in Modeling, Optimization, Characterization and Simulation of Advanced Mechanical Micro/Nano Processes
This Special Issue primarily focuses on enabling technologies that facilitate optimization, designing, Characterization, machining, and manufacturing approaches for micro/nano systems used in different domains: electrical, mechanical, thermal, magnetic, optic, fluidic. This paper collection organizes and disseminates recent research, theories, and practices relevant to areas of micro-system designing, machining, and manufacturing ...read more
Recent Advances in Quantum Nanosystems and AI-driven Nanosystems Design
Micro and nanosystems refer to technologies that involve the design, creation, and manipulation of structures, devices, and systems at the microscopic (micro) and nanoscopic (nano) scales. These systems typically range in size from micrometers (millionths of a meter) to nanometers (billionths of a meter). The primary distinction between micro and ...read more
Related Journals

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Pharmacokinetic Features of the Antiparasitic Macrocyclic Lactones
Current Pharmaceutical Biotechnology Prospects for Caspase Inhibitors
Mini-Reviews in Medicinal Chemistry Innate Immune Surveillance in the Central Nervous System Following Legionella pneumophila Infection
CNS & Neurological Disorders - Drug Targets Nutritional Support in Cancer
Current Nutrition & Food Science Oxidative Stress Targeting Amyloid Beta Accumulation and Clearance in Alzheimer’s Disease: Insight into Pathological Mechanisms and Therapeutic Strategies
Current Psychopharmacology Aquaporin Biology and Nervous System
Current Neuropharmacology Aromatherapy and the Central Nerve System (CNS): Therapeutic Mechanism and its Associated Genes
Current Drug Targets Gene Therapy in Peripheral Nerve Reconstruction Approaches
Current Gene Therapy Thromboembolic Complications in Malignant Haematological Disorders
Current Vascular Pharmacology Is Effective and Safe a Radiochemotherapy Approach in Elderly Cancer Patients? A Review
Anti-Cancer Agents in Medicinal Chemistry Ganoderma lucidum: A Potent Pharmacological Macrofungus
Current Pharmaceutical Biotechnology γ- and δ-Amino Acids: Synthetic Strategies and Relevant Applications
Current Organic Chemistry Assessment of Interleukin-17A, Interleukin-10 and Transforming Growth Factor-Beta1 Serum Titers in Relapsing Remitting Multiple Sclerosis Patients Treated with Avonex, Possible Biomarkers for Treatment Response
CNS & Neurological Disorders - Drug Targets Charcot-Marie-Tooth Disease and Related Hereditary Neuropathies: From Gene Function to Associated Phenotypes
Current Molecular Medicine Intestinal Barrier Dysfunction in Human Pathology and Aging
Current Pharmaceutical Design MicroRNAs in CAG Trinucleotide Repeat Expansion Disorders: an Integrated Review of the Literature
CNS & Neurological Disorders - Drug Targets Update on Intravenous Immunoglobulins (IVIg) Mechanisms of Action and Off- Label use in Autoimmune Diseases
Current Pharmaceutical Design Amyloid Formation in Surfactants and Alcohols: Membrane Mimetics or Structural Switchers?
Current Protein & Peptide Science An In-Silico Investigation of Anti-Chagas Phytochemicals
Current Clinical Pharmacology The Effects of IGF-1 on Trk Expressing DRG Neurons with HIV-gp120- Induced Neurotoxicity
Current HIV Research