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Recent Patents on Biotechnology


ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

Hydrogen Peroxide Enhances Enterokinase-Catalysed Proteolytic Cleavage of Fusion Protein

Author(s): Taian Cui, Yaojun Gao, Cui X. Ang, Chum M. Puah, Bernd Gutte and Yulin Lam

Volume 2, Issue 3, 2008

Page: [188 - 190] Pages: 3

DOI: 10.2174/187220808786240971

Price: $65


The effects of hydrogen peroxide on enterokinase catalysis were studied using several fusion proteins recombinantly produced from E. coli. It was demonstrated that hydrogen peroxide enhanced the rate of enterokinase cleavage reaction, leading to a faster release of the target peptide as discussed in patent WO07149053 [1] . Among the conditions tested, we observed that hydrogen peroxide could exert its effect on the cleavage of fusion proteins over a wide range of pH and temperature. This finding might provide a simple solution for the accelerated enterokinase cleavage of thermolabile fusion proteins at low temperature.

Keywords: Enterokinase cleavage, proteolytic cleavage, fusion proteins, hydrogen peroxide

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