Abstract
A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and - dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
Keywords: High-throughput screening, human umbilical cord blood mononuclear cells, natural products, flow cytometry, immuno-regulatory activities, γ-dodecalactone
Combinatorial Chemistry & High Throughput Screening
Title: A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells
Volume: 9 Issue: 10
Author(s): Chia-Jung Chen, Chia Che Tsai, Jung-Feng Hsieh, Chichen Michael Chien, Tzu-Hua Wu and Shui-Tein Chen
Affiliation:
Keywords: High-throughput screening, human umbilical cord blood mononuclear cells, natural products, flow cytometry, immuno-regulatory activities, γ-dodecalactone
Abstract: A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and - dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
Export Options
About this article
Cite this article as:
Chen Chia-Jung, Che Tsai Chia, Hsieh Jung-Feng, Michael Chien Chichen, Wu Tzu-Hua and Chen Shui-Tein, A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells, Combinatorial Chemistry & High Throughput Screening 2006; 9 (10) . https://dx.doi.org/10.2174/138620706779026033
DOI https://dx.doi.org/10.2174/138620706779026033 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
Call for Papers in Thematic Issues
Artificial Intelligence Methods for Biomedical, Biochemical and Bioinformatics Problems
Recently, a large number of technologies based on artificial intelligence have been developed and applied to solve a diverse range of problems in the areas of biomedical, biochemical and bioinformatics problems. By utilizing powerful computing resources and massive amounts of data, methods based on artificial intelligence can significantly improve the ...read more
Eco-friendly Agents for Biological Control of Pathogenic Diseases
The discovery of an alternative biological approach to disease management includes work on medicinal products derived from natural sources as a starting point for the development of eco-friendly agents for these diseases and the injuries they cause, as well as reducing human contact with hazardous chemicals and their residues. We ...read more
Emerging trends in diseases mechanisms, noble drug targets and therapeutic strategies: focus on immunological and inflammatory disorders
Recently infectious and inflammatory diseases have been a key concern worldwide due to tremendous morbidity and mortality world Wide. Recent, nCOVID-9 pandemic is a good example for the emerging infectious disease outbreak. The world is facing many emerging and re-emerging diseases out breaks at present however, there is huge lack ...read more
Exploring Spectral Graph Theory in Combinatorial Chemistry
Scope of the Thematic Issue: Combinatorial chemistry involves the synthesis and analysis of a large number of diverse compounds simultaneously. Traditional methods rely on brute force experimentation, which can be time-consuming and resource-intensive. Spectral Graph Theory, a branch of mathematics dealing with the properties of graphs in relation to the ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Role of Defensins and Cathelicidin LL37 in Auto-Immune and Auto- Inflammatory Diseases
Current Pharmaceutical Biotechnology Progress in Synthesis and Antitumor Activities of Estradiol-linked Platinum Complex
Mini-Reviews in Medicinal Chemistry Discovery of Selective Probes and Antagonists for G Protein-Coupled Receptors FPR/FPRL1 and GPR30
Current Topics in Medicinal Chemistry Contribution of Catecholamine Reactive Intermediates and Oxidative Stress to the Pathologic Features of Heart Diseases
Current Medicinal Chemistry Cruciferous Plants: Phytochemical Toxicity Versus Cancer Chemoprotection
Mini-Reviews in Medicinal Chemistry Recent Knowledge and New Pharmaceutical Products in Potential Alleviation of Endometriosis
Recent Patents on Inflammation & Allergy Drug Discovery Uncovering New Pharmacological Targets to Treat Neuropathic Pain by Understanding How the Organism Reacts to Nerve Injury
Current Pharmaceutical Design Molecules from Nature: Modulating the Expression of Estrogen Receptor Genes in Breast Cancer Cells
Current Bioactive Compounds The Effects of Newer Beta-Adrenoceptor Antagonists on Vascular Function in Cardiovascular Disease
Current Vascular Pharmacology Oncogene Expression Modulation in Cancer Cell Lines by DNA G-Quadruplex-Interactive Small Molecules
Current Medicinal Chemistry Clinical Application of Human Oocyte Cryopreservation
Reviews on Recent Clinical Trials Kruppel-Like Factors 4 and 5: Unity in Diversity
Current Genomics Estrogen Receptor Beta in Cancer: an Attractive Target for Therapy
Current Pharmaceutical Design Application of RNA Interference for the Control of Female Reproductive Functions
Current Pharmaceutical Design Midkine: A Promising Molecule for Drug Development to Treat Diseases of the Central Nervous System
Current Pharmaceutical Design Cardiovascular Risk Factors: Does Sex Matter?
Current Vascular Pharmacology Marine Derived Anticancer Drugs Targeting Microtubule
Recent Patents on Anti-Cancer Drug Discovery Drug-Loaded Nanocarriers in Tumor Targeted Drug Delivery
Current Biotechnology Drug Transport Across the Placenta
Current Pharmaceutical Biotechnology Connexins as Precocious Markers and Molecular Targets for Chemical and Pharmacological Agents in Carcinogenesis
Current Medicinal Chemistry