Abstract
Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that has been shown to have key roles in cell migration, proliferation and survival. FAK activity can be stimulated in response to several types of extracellular ligands, including components of the extracellular matrix and growth factors, suggesting that FAK is an important integrator of multiple cues in the extracellular milieu. Recently, major progress has been made in understanding the molecular mechanisms regulating FAK activity. In particular, several novel proteins have been identified that can bind to FAK and inhibit its activity and associated cellular functions, including cell motility and invasion. Consistent with its critical functions in signal transduction, FAK also plays a pivotal role in mouse development. The inactivation of FAK in mice results in embryonic lethality around E8.5; this early embryonic lethal phenotype limits the use of the FAK total knockout mouse model for studying FAK function in later embryonic development stages and in adult mice. To overcome this problem, three independent groups created FAK/flox mice and generated several different FAK tissue-specific knockout mice models. Here we summarize the progress that has been made regarding the regulation of FAK-mediated signaling events in cell-based systems and also highlight the in vivo functions of FAK in a number of terminally differentiated cell lineages, including vascular endothelial cells, cardiomyocytes, neuronal cells, keratinocytes and several cancerous cell types.
Keywords: signal transduction, mouse models, tyrosine kinases, cell motility, FAK
Current Protein & Peptide Science
Title: Focal Adhesion Kinase: From In Vitro Studies to Functional Analyses In Vivo
Volume: 12 Issue: 1
Author(s): Xu Peng and Jun-Lin Guan
Affiliation:
Keywords: signal transduction, mouse models, tyrosine kinases, cell motility, FAK
Abstract: Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that has been shown to have key roles in cell migration, proliferation and survival. FAK activity can be stimulated in response to several types of extracellular ligands, including components of the extracellular matrix and growth factors, suggesting that FAK is an important integrator of multiple cues in the extracellular milieu. Recently, major progress has been made in understanding the molecular mechanisms regulating FAK activity. In particular, several novel proteins have been identified that can bind to FAK and inhibit its activity and associated cellular functions, including cell motility and invasion. Consistent with its critical functions in signal transduction, FAK also plays a pivotal role in mouse development. The inactivation of FAK in mice results in embryonic lethality around E8.5; this early embryonic lethal phenotype limits the use of the FAK total knockout mouse model for studying FAK function in later embryonic development stages and in adult mice. To overcome this problem, three independent groups created FAK/flox mice and generated several different FAK tissue-specific knockout mice models. Here we summarize the progress that has been made regarding the regulation of FAK-mediated signaling events in cell-based systems and also highlight the in vivo functions of FAK in a number of terminally differentiated cell lineages, including vascular endothelial cells, cardiomyocytes, neuronal cells, keratinocytes and several cancerous cell types.
Export Options
About this article
Cite this article as:
Peng Xu and Guan Jun-Lin, Focal Adhesion Kinase: From In Vitro Studies to Functional Analyses In Vivo, Current Protein & Peptide Science 2011; 12 (1) . https://dx.doi.org/10.2174/138920311795659452
DOI https://dx.doi.org/10.2174/138920311795659452 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
Call for Papers in Thematic Issues
Advancements in Proteomic and Peptidomic Approaches in Cancer Immunotherapy: Unveiling the Immune Microenvironment
The scope of this thematic issue centers on the integration of proteomic and peptidomic technologies into the field of cancer immunotherapy, with a particular emphasis on exploring the tumor immune microenvironment. This issue aims to gather contributions that illustrate the application of these advanced methodologies in unveiling the complex interplay ...read more
Artificial Intelligence for Protein Research
Protein research, essential for understanding biological processes and creating therapeutics, faces challenges due to the intricate nature of protein structures and functions. Traditional methods are limited in exploring the vast protein sequence space efficiently. Artificial intelligence (AI) and machine learning (ML) offer promising solutions by improving predictions and speeding up ...read more
Nutrition and Metabolism in Musculoskeletal Diseases
The musculoskeletal system consists mainly of cartilage, bone, muscles, tendons, connective tissue and ligaments. Balanced metabolism is of vital importance for the homeostasis of the musculoskeletal system. A series of musculoskeletal diseases (for example, sarcopenia, osteoporosis) are resulted from the dysregulated metabolism of the musculoskeletal system. Furthermore, metabolic diseases (such ...read more
Protein Folding, Aggregation and Liquid-Liquid Phase Separation
Protein folding, misfolding and aggregation remain one of the main problems of interdisciplinary science not only because many questions are still open, but also because they are important from the point of view of practical application. Protein aggregation and formation of fibrillar structures, for example, is a hallmark of a ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Synthesis and Biological Activity of the Pyridine-hexacyclic-steroid Derivative
on a Heart Failure Model
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Perspective on the Role of Four Beta-blockers in Heart Failure
Current Reviews in Clinical and Experimental Pharmacology Cellular and Molecular Mechanisms of Low Cardiac Output Syndrome after Pediatric Cardiac Surgery
Current Vascular Pharmacology Recombinant Human Insulin-Like Growth Factor-1: A New Cardiovascular Disease Treatment Option?
Cardiovascular & Hematological Agents in Medicinal Chemistry Anabolic Androgenic Steroid (AAS) Related Deaths: Autoptic, Histopathological and Toxicological Findings
Current Neuropharmacology Current Advances in the Synthesis and Antitumoral Activity of SIRT1-2 Inhibitors by Modulation of p53 and Pro-Apoptotic Proteins
Current Medicinal Chemistry Pharmacological Profile and Pharmacogenomics of Anti-Cancer Drugs Used for Targeted Therapy
Current Cancer Drug Targets Editorial: Perioperative Pharmacological Management: From Prescription Medications to Dietary Supplements
Current Clinical Pharmacology From Amino Acids to Proteins as Targets for Metal-based Drugs
Current Drug Metabolism Gender Differences in the Effects of Angiotensin Receptor Blockers on Cardiovascular Disease
Current Pharmaceutical Design Exosomal MiR-29a in Cardiomyocytes Induced by Angiotensin II Regulates Cardiac Microvascular Endothelial Cell Proliferation, Migration and Angiogenesis by Targeting VEGFA
Current Gene Therapy Ghrelin and Cardiovascular Diseases
Current Cardiology Reviews Fetal Origins of Cardiovascular Disease
Current Cardiology Reviews Cell Sheet-Based Myocardial Tissue Engineering: New Hope for Damaged Heart Rescue
Current Pharmaceutical Design Energetic Metabolic Roles in Pulmonary Arterial Hypertension and Right Ventricular Remodeling
Current Pharmaceutical Design Influence of a High-Fat Diet on Cardiac iNOS in Female Rats
Current Vascular Pharmacology Endotherapia
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Life-history Constraints on the Mechanisms that Control the Rate of ROS Production
Current Genomics Development of Inhibitors of the Aspartyl Protease Renin for the Treatment of Hypertension
Current Protein & Peptide Science Effectors of Fatty Acid Oxidation Reduction: Promising New Anti-Ischaemic Agents
Current Pharmaceutical Design